2.1.1.312: 25S rRNA (uracil2843-N3)-methyltransferase
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For detailed information about 25S rRNA (uracil2843-N3)-methyltransferase, go to the full flat file.
Reaction
Synonyms
BMT6, YLR063W
ECTree
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Cloned
Cloned on EC 2.1.1.312 - 25S rRNA (uracil2843-N3)-methyltransferase
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plasmid pSH25 carrying N terminal 6xHis-Smt3tagged Bmt6, is constructed using pPK591 by gap repair. For overexpression, BL21 (DE3) cells are transformed with isolated plasmids and grown in LB medium containing 100 mg/ml ampicillin at 37°C to an OD600 of 0.6