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2.1.1.296: methyltransferase cap2

This is an abbreviated version!
For detailed information about methyltransferase cap2, go to the full flat file.

Word Map on EC 2.1.1.296

Reaction

S-adenosyl-L-methionine
+
a 5'-(N7-methyl 5'-triphosphoguanosine)-(2'-O-methyl-ribonucleotide)-(ribonucleotide)-[mRNA]
=
S-adenosyl-L-homocysteine
 +
a 5'-(N7-methyl 5'-triphosphoguanosine)-(2'-O-methyl-ribonucleotide)-(2'-O-methyl-ribonucleotide)-[mRNA]

Synonyms

AFT, cap2, cap2-MTase, CMTr2, FLJ11171, FTSJD1, hMTr2, mRNA (nucleoside-2'-O)-methyltransferase, MT48, MT57, MTR2, TbMT48, TbMT57

ECTree

     2 Transferases
         2.1 Transferring one-carbon groups
             2.1.1 Methyltransferases
                2.1.1.296 methyltransferase cap2

Cloned

Cloned on EC 2.1.1.296 - methyltransferase cap2

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
gene cap2, quantitative RT-PCR expression analysis
gene CMTR2, recombinant overexpression of wild-type and mutant enzymes in HEK-293 cells
gene HMTR2 or FTSJD1, phylogenetic tree, functional overexpression of the cap2 MTase hMTr2 and of c-myc-tagged enzyme in MCF7 cells
primer extension analysis, functional expression observed of the MT48 gene tagged at the C-terminus with an HA epitope in mt48-/- cells
the mt57-/- mutant cells are complemented with the triple MT57 mutant W62D/G63D/Q64D and with MT57 containing the K266A mutation, both mutants do not reestablish the +5 primer extension stop to wild-type levels