2.1.1.292: carminomycin 4-O-methyltransferase

This is an abbreviated version!
For detailed information about carminomycin 4-O-methyltransferase, go to the full flat file.

Word Map on EC 2.1.1.292

2.1.1.292

methyltransferases

sam-dependent

s-adenosyl-l-homocysteine

daunorubicin

peucetius

s-adenosyl-l-methionine-dependent

synthesis

Reaction

Synonyms

4-O-methyltransferase, anthracycline 4-O-methyltransferase, anthracycline methyltransferase, carminomycin 4-OMT, class I methyltransferase, COMT, DauK, DnrK

ECTree

2 Transferases

2.1 Transferring one-carbon groups

2.1.1 Methyltransferases

2.1.1.292 carminomycin 4-O-methyltransferase

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Results	in table
5	AA Sequence
1	Application
7	Cloned(Commentary)
4	Cofactor
2	Crystallization (Commentary)
10	General Information
3	Inhibitors
3	kcat/KM [mM/s]
6	KM Value [mM]
1	Metals/Ions
3	Molecular Weight [Da]
9	Natural Substrates/ Products (Substrates)
11	Organism
5	Pathway
57	PDB ID
3	pH Optimum
1	pH Range
6	Protein Variants
3	Purification (Commentary)
1	Reaction
11	Reference
2	Source Tissue
1	Specific Activity [micromol/min/mg]
24	Substrates and Products (Substrate)
3	Subunits
18	Synonyms
1	Systematic Name
2	Temperature Optimum [°C]
1	Temperature Range [°C]
3	Turnover Number [1/s]

Natural Substrates Products

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Natural Substrates Products on EC 2.1.1.292 - carminomycin 4-O-methyltransferase

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S-adenosyl-L-methionine + 11-deoxy-beta-rhodomycin T

S-adenosyl-L-homocysteine + 4-O-methyl-11-deoxy-beta-rhodomycin T

Streptomyces peucetius

Q06528

758220

S-adenosyl-L-methionine + 13-dihydrocarminomycin

S-adenosyl-L-homocysteine + daunorubicinol

Streptomyces sp.

terminal enzyme in the daunomycin biosynthesis pathway

722880

S-adenosyl-L-methionine + 15-demethoxy-aclacinomycin T

S-adenosyl-L-homocysteine + 4-O-methyl-15-decarboxyaclacinomycin T

Streptomyces peucetius

Q06528

758220

S-adenosyl-L-methionine + carminomycin

S-adenosyl-L-homocysteine + daunomycin

Streptomyces sp.

Q55216

with the corresponding aglycones, carminomycinone and 13-dihydrocarminomycinone, as substrates, no methylated products are detected. Other 4-hydroxyanthracyclines such as aklavin and aclacinomycin A, and 4-hydroxyanthracyclinones such as erhodomycinone and aklavinone, are not substrates for the 4-O-methyltransferase. These reaction specificities indicate that glycosylation of the anthracyclinone molecule must occur before 4-O-methylation, which means that 4-O-methylation of carminomycin is probably the terminal step in the biosynthesis of daunomycin, and that daunomycinone is not an intermediate in the pathway

722879

S-adenosyl-L-methionine + carminomycin

S-adenosyl-L-homocysteine + daunorubicin

2 entries

additional information

Streptomyces peucetius

Q06528

the enzyme possesses rather relaxed substrate specificity in regard to modifications in the polyaromatic anthracycline ring system, but it is quite specific with respect to the length of the carbohydrate chain at C-7, accepting only monoglycosides. In addition, DnrK has even been shown to be able to methylate various flavonoids. Discovery of a 10-decarboxylation activity of DnrK

758220