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2.1.1.292: carminomycin 4-O-methyltransferase

This is an abbreviated version!
For detailed information about carminomycin 4-O-methyltransferase, go to the full flat file.

Word Map on EC 2.1.1.292

Reaction

S-adenosyl-L-methionine
+
carminomycin
=
S-adenosyl-L-homocysteine
 +
daunorubicin

Synonyms

4-O-methyltransferase, anthracycline 4-O-methyltransferase, anthracycline methyltransferase, carminomycin 4-OMT, class I methyltransferase, COMT, DauK, DnrK

ECTree

     2 Transferases
         2.1 Transferring one-carbon groups
             2.1.1 Methyltransferases
                2.1.1.292 carminomycin 4-O-methyltransferase

Cloned

Cloned on EC 2.1.1.292 - carminomycin 4-O-methyltransferase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
expression in Streptomyces violaceus pMK100 (epelmycin producer). The transformant produces the hybrid anthracycline antibiotic 7-O-L-rhodosaminyl-4-O-methyl-epsilon-rhodomycinone (4-O-methylepelmycin D) together with host epelmycins when cultured in antibiotic production medium in the presence of thiostrepton. Attempts on production of hybrid 4-O-methylaclarubicin and 4-O-methyl-1-deoxyobelmycin by the transformants of aclarubicin and 1-deoxyobelmycin producers are unsuccessful
-
gene dnrK, phylogenetic analysis
gene dnrK, recombinant expression of N-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3)