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2.1.1.282: tRNAPhe 7-[(3-amino-3-carboxypropyl)-4-demethylwyosine37-N4]-methyltransferase

This is an abbreviated version!
For detailed information about tRNAPhe 7-[(3-amino-3-carboxypropyl)-4-demethylwyosine37-N4]-methyltransferase, go to the full flat file.

Reaction

S-adenosyl-L-methionine
+
7-[(3S)-(3-amino-3-carboxypropyl)]-4-demethylwyosine37 in tRNAPhe
=
S-adenosyl-L-homocysteine
 +
7-[(3S)-(3-amino-3-carboxypropyl)]wyosine37 in tRNAPhe

Synonyms

aTrm5a methyltransferase, aTrm5a-type enzyme, aTrm5a/Taw22-like enzyme, More, NEQ228, PAB2272, PaTrm5a, SSO2439, Taw21, TAW22, Trm5a, Trm5a/Taw22-like enzyme, tRNAPhe:imG2 methyltransferase, TYW3, YGL050w

ECTree

     2 Transferases
         2.1 Transferring one-carbon groups
             2.1.1 Methyltransferases
                2.1.1.282 tRNAPhe 7-[(3-amino-3-carboxypropyl)-4-demethylwyosine37-N4]-methyltransferase

Cloned

Cloned on EC 2.1.1.282 - tRNAPhe 7-[(3-amino-3-carboxypropyl)-4-demethylwyosine37-N4]-methyltransferase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
gene trm5a, recombinant expression of wild-type and mutant enzymes
gene trm5a, sequence comparisons, recombinant expression of N-terminally His6-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)
hexahistidine-tagged enzyme is expressed in Saccharomyces cerevisiae
recombinant expression of His-tagged enzyme
recombinant expression of His-tagged wild-type and mutant enzymes