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2.1.1.281: phenylpyruvate C3-methyltransferase

This is an abbreviated version!
For detailed information about phenylpyruvate C3-methyltransferase, go to the full flat file.

Reaction

S-adenosyl-L-methionine
+
3-phenylpyruvate
=
S-adenosyl-L-homocysteine
+
(3S)-2-oxo-3-phenylbutanoate

Synonyms

C-MT, ELQ87_35210, MppJ, MT SgvM, phenylpyruvate Cbeta-methyltransferase, phenylpyruvate methyltransferase, S-adenosylmethionine-dependent methyltransferase, SgvM

ECTree

     2 Transferases
         2.1 Transferring one-carbon groups
             2.1.1 Methyltransferases
                2.1.1.281 phenylpyruvate C3-methyltransferase

Crystallization

Crystallization on EC 2.1.1.281 - phenylpyruvate C3-methyltransferase

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CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
purified recombinant His6-tagged enzyme, in apoform or in complex with S-adenosyl-L-methionine and phenylpyruvate, hanging drop vapour diffusion technique, mixing of 0.001 ml of 24 mg/ml protein in 50 mM HEPES, pH 7.5, 100 mM CaCl2, with 0.001 ml of reservoir solution containing 16% PEG 3350, 200 mM NaI, 20°C, 10 days, X-ray diffraction structure determination and analysis at 2.0-2.5 A resolution, molecular replacement. From the collected crystal diffraction data four additional structures of MppJ in complex with phenlpyruvate, 4-hydroxyphenylpyruvate, S-adenosyl-L-methionine and phenylpyruvate or S-adenosyl-L-homocysteine and methylphenylpyruvate, although crystals of the apo form remain unobtainable