2.1.1.280: selenocysteine Se-methyltransferase
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For detailed information about selenocysteine Se-methyltransferase, go to the full flat file.
Word Map on EC 2.1.1.280
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2.1.1.280
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selenium
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selenate
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astragalus
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se-methylselenocysteine
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hyperaccumulator
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bisulcatus
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brassica
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oleracea
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mesecys
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homocysteine
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methylselenocysteine
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phytoremediation
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sulfurylase
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non-accumulating
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broccoli
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mustard
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agriculture
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juncea
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italica
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brassicaceae
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organoselenium
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s-methylmethionine
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nonprotein
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chemopreventative
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biofortification
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synthesis
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fabaceae
- 2.1.1.280
- selenium
- selenate
- astragalus
- se-methylselenocysteine
-
hyperaccumulator
- bisulcatus
-
brassica
- oleracea
-
mesecys
- homocysteine
-
methylselenocysteine
-
phytoremediation
-
sulfurylase
-
non-accumulating
- broccoli
- mustard
- agriculture
- juncea
- italica
- brassicaceae
-
organoselenium
- s-methylmethionine
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nonprotein
-
chemopreventative
-
biofortification
- synthesis
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fabaceae
Reaction
Synonyms
BjSMT, BoSMT, Hcy methyltransferase, SEcMT, SeCys-methyltransferase, SeCys-MT, selenocysteine methyltransferase, SMT, smtA, YagD, YagD protein
ECTree
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Engineering
Engineering on EC 2.1.1.280 - selenocysteine Se-methyltransferase
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A184T
mutant enzyme gains the ability to methylate L-homocysteine
additional information
the but lacks the selenocysteine methyltransferase activity in vitro, explaining why little or no detectable levels of Se-methylselenocysteine accumulation are observed in the non-accumulator plant. Sequence analysis reveals that the selenocysteine methyltransferase from all plant that accumulate selenium contain a glycine residue at position 24, whereas the plants that do not accumulate selenium possess an alanine residue. A phenylalanine residue at position 148 in the accumulator selenocysteine methyltransferases is replaced by a tyrosine residue in non-accumulators. Thr334 is common among the Se accumulator species is lacking in the non-accumulator selenocysteine methyltransferase sequences and it appears as a complete deletion of the Thr334 codon of the DNA sequence
additional information
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the but lacks the selenocysteine methyltransferase activity in vitro, explaining why little or no detectable levels of Se-methylselenocysteine accumulation are observed in the non-accumulator plant. Sequence analysis reveals that the selenocysteine methyltransferase from all plant that accumulate selenium contain a glycine residue at position 24, whereas the plants that do not accumulate selenium possess an alanine residue. A phenylalanine residue at position 148 in the accumulator selenocysteine methyltransferases is replaced by a tyrosine residue in non-accumulators. Thr334 is common among the Se accumulator species is lacking in the non-accumulator selenocysteine methyltransferase sequences and it appears as a complete deletion of the Thr334 codon of the DNA sequence
additional information
transgenic expression in Nicotiana tabacum. When plants are watered with 200 microM selenate, overexpression of a selenocysteine methyltransferase transgene causes a 2- to 4fold increase in Se accumulation resulting in increased numbers of leaf lesions and areas of necrosis, production of methylselenocysteine up to 20% of total Se and generation of volatile dimethyl diselenide derived directly from methylselenocysteine. Despite the greatly increased accumulation of total Se, this does not result in increased Se toxicity effects on growth. Overexpression of ATP sulfurylase does not increase Se accumulation from selenate. Lines overexpressing both ATP sulfurylase and selenocysteine methyltransferase do not show a further increase in total Se accumulation or in leaf toxicity symptoms relative to overexpression of selenocysteine methyltransferase alone, but direct a greater proportion of Se into methylselenocysteine
additional information
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overexpression of BjSMT in tobacco substantially enhances tolerance to selenite stress manifested as significantly higher fresh weight, plant height, and chlorophyll content than control plants. Transgenic plants exhibited low glutathione peroxidase activity in response to a lower dose of selenite stress (with a higher dose of selenite stress resulting in a high activity response) compared with the controls. The BjSMT-transformed tobacco plants accumulate a high level of Se upon selenite stress, and the plants also have significantly increased MeSeCys production potential in their leaves
additional information
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overexpression of SMT decreases the negative effect of selenium on sulforaphane synthesis, while knockdown of SMT by RNAi enhances the negative effect
additional information
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overexpression of SMT decreases the negative effect of selenium on sulforaphane synthesis, while knockdown of SMT by RNAi enhances the negative effect
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additional information
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transgenic expression in arabidopsis thaliana. After high zinc stress, the transgenic plants over-expressing SmtA show higher survival rate than the wild type. Over-expression of SmtA in Arabidopsis increases the activities of superoxide dismutase and peroxidase, and enhances the tolerance to zinc stress