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2.1.1.280: selenocysteine Se-methyltransferase

This is an abbreviated version!
For detailed information about selenocysteine Se-methyltransferase, go to the full flat file.

Word Map on EC 2.1.1.280

Reaction

S-methyl-L-methionine
+
L-selenocysteine
=
L-methionine
 +
Se-methyl-L-selenocysteine

Synonyms

BjSMT, BoSMT, Hcy methyltransferase, SEcMT, SeCys-methyltransferase, SeCys-MT, selenocysteine methyltransferase, SMT, smtA, YagD, YagD protein

ECTree

     2 Transferases
         2.1 Transferring one-carbon groups
             2.1.1 Methyltransferases
                2.1.1.280 selenocysteine Se-methyltransferase

Cloned

Cloned on EC 2.1.1.280 - selenocysteine Se-methyltransferase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
BoSMT overexpression in Escherichia coli strain MTD123
cloned into plasmid pT7-7 and overexpression in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli
expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli BL21Star (DE3) cells
expressed in Escherichia coli Top10 cells
expressed in Indian mustard plants
-
expression in Arabidopsis thaliana
-
expression in Nicotiana tabacum
gene SMT, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis and tree, recombinant expression of GFP-tagged enzyme under control of 35S promoter in Nicotiana tabacum, quantitative real-time PCR enzyme expression analysis
-
gene SMT, quantitative real-time PCR enzyme expression analysis
-
gene SMT1, expression in Solanum lycopersicum, the transgenic plants are provided with selenite or selenate to the roots during fruit development, they accumulate MeSeCys in the fruits
-
generation of enzyme overexpressing lines
-
overproduction in Arabidopsis thaliana leads to increased Se accumulation and selenite resistance
-
pUKE vector used for cloning of cDNA sequence coding for the SeCys-methyltransferase from Astragalus bisulcatus in Escherichia coli