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2.1.1.28: phenylethanolamine N-methyltransferase

This is an abbreviated version!
For detailed information about phenylethanolamine N-methyltransferase, go to the full flat file.

Word Map on EC 2.1.1.28

Reaction

S-adenosyl-L-methionine
+
phenylethanolamine
=
S-adenosyl-L-homocysteine
 +
N-Methylphenylethanolamine

Synonyms

hPNMT, methyltransferase, noradrenaline N-, NMT, noradrenalin methyltransferase, noradrenalin N-methyltransferase, noradrenaline N-methyltransferase, norepinephrin N-methyltransferase, norepinephrine methyltransferase, norepinephrine N-methyltransferase, phenethanolamine methyltransferase, phenethanolamine N-methyltransferase, PNMT

ECTree

     2 Transferases
         2.1 Transferring one-carbon groups
             2.1.1 Methyltransferases
                2.1.1.28 phenylethanolamine N-methyltransferase

Subunits

Subunits on EC 2.1.1.28 - phenylethanolamine N-methyltransferase

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SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
dimer
-
gel filtration reveals the presence of two species at elution volumes consistent with monomeric and dimeric human phenylethanolamine N-methyltransferase. The more prominent peak corresponds to the dimer form. The amount of dimer can be reduced either by using a more elute concentration of the protein or by the addition of 0.5 mM DTT to the running buffer. SDS-PAGE can not distinguish between the two forms. Native PAGE clearly distinguishes between the two forms of human phenylethanolamine N-methyltransferase. Crystals from the dimer fraction grow faster. Monomer and dimer human phenylethanolamine N-methyltransferase have similar kinetic constatns. The monomer-dimer equilibruim in analytical ultracentrifugation for the dimer frations of phenylethanolamine N-methyltransferase-His and C48A phenylethanolamine N-methyltransferase is 10fold lower than for the monomer fractions (Kd 35-64 microM and 305-551 microM, respectively).
monomer