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2.1.1.28: phenylethanolamine N-methyltransferase

This is an abbreviated version!
For detailed information about phenylethanolamine N-methyltransferase, go to the full flat file.

Word Map on EC 2.1.1.28

Reaction

S-adenosyl-L-methionine
+
phenylethanolamine
=
S-adenosyl-L-homocysteine
+
N-Methylphenylethanolamine

Synonyms

hPNMT, methyltransferase, noradrenaline N-, NMT, noradrenalin methyltransferase, noradrenalin N-methyltransferase, noradrenaline N-methyltransferase, norepinephrin N-methyltransferase, norepinephrine methyltransferase, norepinephrine N-methyltransferase, phenethanolamine methyltransferase, phenethanolamine N-methyltransferase, PNMT

ECTree

     2 Transferases
         2.1 Transferring one-carbon groups
             2.1.1 Methyltransferases
                2.1.1.28 phenylethanolamine N-methyltransferase

Purification

Purification on EC 2.1.1.28 - phenylethanolamine N-methyltransferase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
5 charge isoenzymes: E-1, E-2, E-3, E-4, E-5
-
6X-histidine tagged phenylethanolamine N-methyltransferase purified on a 10 ml column and with gel filtration. Non-tagged phenylethanolamine N-methyltransferase is purified with DEAE-Sepharose and gel filtration.
-
at least 4 active charged isoenzymes
-
enzyme form H-1 and H-2
-
His-Select HC nickel affninity column and gel filtration
recombinant C-terminally His6-tagged wild-type and mutant enzymes
-
using Ni-NTA chromatography
-