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2.1.1.274: salicylate 1-O-methyltransferase

This is an abbreviated version!
For detailed information about salicylate 1-O-methyltransferase, go to the full flat file.

Word Map on EC 2.1.1.274

Reaction

S-adenosyl-L-methionine
+
salicylate
=
S-adenosyl-L-homocysteine
+
methyl salicylate

Synonyms

AlBSMT1, AtBSMT1, benzoate-salicylate methyltransferase, benzoic acid/SA methyltransferase, benzoic acid/salicylic acid carboxyl methyltransferase, benzoic acid/salicylic acid carboxyl methyltransferase 1, benzoic acid/salicylic acid methyltransferase, benzoic acid/salicylic acidcarboxyl methyltransferase, BSMT, BSMT1, class II SABATH protein, CsSAMT, LcSAMT, More, PaSABATH2, PaSAMT, PbBSMT methyltransferase, PtSABATH4, S-adenosyl-l-methionine: salicylic acid carboxyl methyl-transferase, S-adenosyl-L-methionine: salicylic acid carboxyl methyltransferase, S-adenosyl-L-methionine:salicylic acid carboxyl methyltransferase, SA carboxyl methyltransferase, SA methyl transferase, SA methyltransferase, SA MTase, SABATH methyltransferase 4, SABATH4, salicylic acid carboxyl methyltransferase, salicylic acid carboxyl methyltransferase-like, salicylic acid methyl transferase, salicylic acid methyltransferase, salicylicacid carboxyl methyl transferase, SAMT, SAMT1

ECTree

     2 Transferases
         2.1 Transferring one-carbon groups
             2.1.1 Methyltransferases
                2.1.1.274 salicylate 1-O-methyltransferase

Cloned

Cloned on EC 2.1.1.274 - salicylate 1-O-methyltransferase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
Clarkia breweri SAMT cloned into expression vector pET28a(+) and construct transformed into Escherichia coli BL21(DE3) cells
coding regions of AlBSMT1 ligated into pCRT7/CT-TOPO TA vector for functional expression in Escherichia coli
coding regions of AtBSMT1 ligated into pCRT7/CT-TOPO TA vector for functional expression in Escherichia coli
expressed in Escherichia coli BL21(DE3) CodonPlus cells
-
expressed in Escherichia coli BL21-AI cells
-
gene BSMT, cloning in Escherichia coli, Arabidopsis thaliana plants are transformed using the floral dip method via Agrobacterium tumefaciens AGL1, recombinant BSMT enzyme expression in Arabidopsis thaliana under the control of a dexamethasone-inducible promoter leading to chlorosis and altered host susceptibility, induced transgenic plants are not able to support large galls and have a brownish appearance of some clubs. The methylester of SA (MeSA) is transported from clubbed Arabidopsis roots to leaves, as shown using heavy isotope-labelled MeSA, and is emitted only from leaves of infected plants, semi- and quantitative RT-PCR expression analysis
gene BSMT, real-time PCR enzyme expression analysis in Plasmodiophora brassicae-infected Brassica oleracea lines
gene BSMT, recombinant expression of 35S::PbBSMT in planta in Arabidopsis thaliana reduces salicylate (SA) levels and increases susceptibility to clubroot, Plasmodiophora brassicae
gene LiBSMT, DNA and amino acid sequence determination and analysis, sequence comparison and phylogenetic analysis and tree, quantitative real-time PCR expression analysis, recombinant expression of N-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3)
gene NbSAMT, quantitative real-time PCR enzyme expression analysis
-
gene PbBSMT, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) codon plus
gene SABATH4, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, overview, recombinant expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)
gene SAMT, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, functional recombinant expression in Escherichia coli, and functional recombinant expression in transgenic Nicotiana tabacum plants via Agrobacterium tunefaciens strain EHA105 transfection method, overexpression of LcSAMT gene markedly enhances the methylsalicylate content and reduces the accumulation of salicylate in transgenic tobacco plants
gene SAMT, sequence comparisons, regulatory elements in the cloned CsSAMT promoter sequence, real-time quantitative PCR enzyme expression analysis, recombinant expression in Escherichia coli and in Saccharomyces cerevisiae
genes PsSABATH2, DNA and amino acid sequence determination and analysis, sequence comparisons, genetic organization and localization of SABATH genes PsSABATH1-10 in the genome of Picea abies, overview. Sequence similarities among PaSABATHs range from 49% to 91%. Most PaSABATH genes have three introns with the exception of PaSABATH4, PaSABATH5 and PaSABATH10, each of which contains two introns. Phylogenetic analysis and tree
-
recombinant expression in Escherichia coli strain BL21(DE3)
SAMT cDNA expressed with pET-11a/pET-28 vector in Escherichia coli which synthesizes a functional non-tagged/His-tagged SAMT protein
subcloned into expression vector pET-28a, containing an N-terminal polyhistidine 6-His-tag, and expressed in Escherichia coli