2.1.1.261: 4-dimethylallyltryptophan N-methyltransferase
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For detailed information about 4-dimethylallyltryptophan N-methyltransferase, go to the full flat file.
Reaction
Synonyms
easF, fgaMT
ECTree
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Cloned
Cloned on EC 2.1.1.261 - 4-dimethylallyltryptophan N-methyltransferase
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gene easF, a fragment of genomic DNA containing dmaW, easF, easE, and easC is amplified from the human-pathogenic, ergot alkaloid-producing fungus Aspergillus fumigatus and transformed into Aspergillus nidulans strain FGSC A767, a model fungus that does not contain any of the ergot alkaloid synthesis genes. Transformed Aspergillus nidulans strains produces chanoclavine-I and an earlier pathway intermediate
gene fgaMT, DNA and amino acid sequence determination and analysis, overexpression of the soluble His6-tagged enzyme in Escherichia coli strain XL-1 blue MRF-