2.1.1.257: tRNA (pseudouridine54-N1)-methyltransferase
This is an abbreviated version!
For detailed information about tRNA (pseudouridine54-N1)-methyltransferase, go to the full flat file.
Word Map on EC 2.1.1.257
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2.1.1.257
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methanocaldococcus
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jannaschii
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knot
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haloferax
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spout
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volcanii
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hypermodified
- 2.1.1.257
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methanocaldococcus
- jannaschii
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knot
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haloferax
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spout
- volcanii
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hypermodified
Reaction
Synonyms
COG 1901, COG1901, DUF358, DUF358/Mj1640, Emg1, Hvo_1989, m1-pseudouridine methyltransferase, m1G, m1PSI methyltransferase, M3U, Mja_1640, MjNep1, Nep1, Pfam DUF358, pseudouridine N1-specific methyltransferase, pseudouridine-N1-specific methyltransferase, ribosome assembly factor Nep1, SAM-dependent N1-pseudouridine methyltransferase, TrmY, TrmY protein
ECTree
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Substrates Products
Substrates Products on EC 2.1.1.257 - tRNA (pseudouridine54-N1)-methyltransferase
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REACTION DIAGRAM
S-adenosyl-L-methionine + pseudouridine in 5'-GAUPsiCAACGCC-3'
S-adenosyl-L-homocysteine + N1-methylpseudouridine in 5'-GAUmethylPsiCAACGCC-3'
S-adenosyl-L-methionine + pseudouridine in 5'-PsiCAACGCC-3'
S-adenosyl-L-homocysteine + N1-methylpseudouridine in 5'-methylPsiCAACGCC-3'
S-adenosyl-L-methionine + pseudouridine in 5'-UPsiCAACGC--3'
S-adenosyl-L-homocysteine + N1-methylpseudouridine in 5'-UmethylPsiCAACGC--3'
S-adenosyl-L-methionine + pseudouridine in 5'-UPsiCAACGCC-3'
S-adenosyl-L-homocysteine + N1-methylpseudouridine in 5'-UmethylPsiCAACGCC-3'
S-adenosyl-L-methionine + pseudouridine54 in elongator tRNAMet
S-adenosyl-L-homocysteine + N1-methylpseudouridine54 in elongator tRNAMet
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S-adenosyl-L-methionine + pseudouridine54 in tRNA
S-adenosyl-L-homocysteine + N1-methylpseudouridine54 in tRNA
S-adenosyl-L-methionine + pseudouridine54 in tRNATrp
S-adenosyl-L-homocysteine + N1-methylpseudouridine54 in tRNATrp
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S-adenosyl-L-homocysteine + N1-methylpseudouridine in 5'-GAUmethylPsiCAACGCC-3'
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S-adenosyl-L-methionine + pseudouridine in 5'-GAUPsiCAACGCC-3'
S-adenosyl-L-homocysteine + N1-methylpseudouridine in 5'-GAUmethylPsiCAACGCC-3'
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?
S-adenosyl-L-homocysteine + N1-methylpseudouridine in 5'-methylPsiCAACGCC-3'
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?
S-adenosyl-L-methionine + pseudouridine in 5'-PsiCAACGCC-3'
S-adenosyl-L-homocysteine + N1-methylpseudouridine in 5'-methylPsiCAACGCC-3'
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?
S-adenosyl-L-homocysteine + N1-methylpseudouridine in 5'-UmethylPsiCAACGC--3'
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?
S-adenosyl-L-methionine + pseudouridine in 5'-UPsiCAACGC--3'
S-adenosyl-L-homocysteine + N1-methylpseudouridine in 5'-UmethylPsiCAACGC--3'
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?
S-adenosyl-L-homocysteine + N1-methylpseudouridine in 5'-UmethylPsiCAACGCC-3'
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?
S-adenosyl-L-methionine + pseudouridine in 5'-UPsiCAACGCC-3'
S-adenosyl-L-homocysteine + N1-methylpseudouridine in 5'-UmethylPsiCAACGCC-3'
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S-adenosyl-L-homocysteine + N1-methylpseudouridine54 in tRNA
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S-adenosyl-L-methionine + pseudouridine54 in tRNA
S-adenosyl-L-homocysteine + N1-methylpseudouridine54 in tRNA
methylation of pseudouridine PSI at position 54 of tRNA, producing m1PSI, in vitro pseudouridylated tRNATrp is used as a substrate
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S-adenosyl-L-methionine + pseudouridine54 in tRNA
S-adenosyl-L-homocysteine + N1-methylpseudouridine54 in tRNA
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?
S-adenosyl-L-methionine + pseudouridine54 in tRNA
S-adenosyl-L-homocysteine + N1-methylpseudouridine54 in tRNA
methylation of pseudouridine PSI at position 54 of tRNA, producing m1PSI, in vitro pseudouridylated tRNATrp is used as a substrate
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?
S-adenosyl-L-methionine + pseudouridine54 in tRNA
S-adenosyl-L-homocysteine + N1-methylpseudouridine54 in tRNA
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?
S-adenosyl-L-methionine + pseudouridine54 in tRNA
S-adenosyl-L-homocysteine + N1-methylpseudouridine54 in tRNA
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?
S-adenosyl-L-methionine + pseudouridine54 in tRNA
S-adenosyl-L-homocysteine + N1-methylpseudouridine54 in tRNA
methylation of pseudouridine PSI at position 54 of tRNA, producing m1PSI, in vitro pseudouridylated tRNATrp is used as a substrate
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?
S-adenosyl-L-methionine + pseudouridine54 in tRNA
S-adenosyl-L-homocysteine + N1-methylpseudouridine54 in tRNA
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?
S-adenosyl-L-methionine + pseudouridine54 in tRNA
S-adenosyl-L-homocysteine + N1-methylpseudouridine54 in tRNA
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?
S-adenosyl-L-methionine + pseudouridine54 in tRNA
S-adenosyl-L-homocysteine + N1-methylpseudouridine54 in tRNA
methylation of pseudouridine PSI at position 54 of tRNA, in vitro and in vivo reaction, producing m1PSI, in vitro pseudouridylated tRNATrp is used as a substrate
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enzymatic post-transcriptional modifications of selected uridines in tRNA, secondary structure, U54 targets within the highly conserved 7-nt TPSI-loop, overview
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additional information
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enzymatic post-transcriptional modifications of selected uridines in tRNA, secondary structure, U54 targets within the highly conserved 7-nt TPSI-loop, overview
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additional information
?
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enzymatic post-transcriptional modifications of selected uridines in tRNA, secondary structure, U54 targets within the highly conserved 7-nt TPSI-loop, overview
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?
additional information
?
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enzymatic post-transcriptional modifications of selected uridines in tRNA, secondary structure, U54 targets within the highly conserved 7-nt TPSI-loop, overview
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?
additional information
?
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enzymatic post-transcriptional modifications of selected uridines in tRNA, secondary structure, U54 targets within the highly conserved 7-nt TPSI-loop, overview
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-
?
additional information
?
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enzymatic post-transcriptional modifications of selected uridines in tRNA, secondary structure, U54 targets within the highly conserved 7-nt TPSI-loop, overview
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-
?
additional information
?
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enzymatic post-transcriptional modifications of selected uridines in tRNA, secondary structure, U54 targets within the highly conserved 7-nt TPSI-loop, overview
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?
additional information
?
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Mja_1640 methylates T-arm resembling tRNAs in vitro. Using synthetic 17 mer RNAs resembling the T-arm of Methanocaldococcus jannaschii tRNATrp that contains a pseudouridine at either position 54, 55, or both, the capability of MjNep1 to methylate these substrates is shown, MjNep1 methylates the substrate with a pseudouridine at position 55 with reasonable efficiency, but markedly slower than its native small ribosomal subunit rRNA-derived substrate, and no methylation is observed for the substrate with pseudouridine at position 54, MjNep1 is incapable of modifying mature H. volcanii tRNAs. MjNep1 is apparently not the pseudouridine-N1 methyltransferase responsible for the tRNA-modification at position 54 in archaeal tRNAs
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additional information
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Mja_1640 methylates T-arm resembling tRNAs in vitro. Using synthetic 17 mer RNAs resembling the T-arm of Methanocaldococcus jannaschii tRNATrp that contains a pseudouridine at either position 54, 55, or both, the capability of MjNep1 to methylate these substrates is shown, MjNep1 methylates the substrate with a pseudouridine at position 55 with reasonable efficiency, but markedly slower than its native small ribosomal subunit rRNA-derived substrate, and no methylation is observed for the substrate with pseudouridine at position 54, MjNep1 is incapable of modifying mature H. volcanii tRNAs. MjNep1 is apparently not the pseudouridine-N1 methyltransferase responsible for the tRNA-modification at position 54 in archaeal tRNAs
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additional information
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SAM-binding pocket Asp157 or Glu183 from its own monomer or Ser43 from the associate monomer probably plays the catalytic role for RNA methylation, RNA-binding residues and putative active site, overview
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additional information
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SAM-binding pocket Asp157 or Glu183 from its own monomer or Ser43 from the associate monomer probably plays the catalytic role for RNA methylation, RNA-binding residues and putative active site, overview
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additional information
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substrate specificity analysis by NMR binding and affinity study for RNA, overview. The enzyme is highly specific for pseudouridine. Methanocaldococcus jannaschii Nep1 binds to the yeast Nep1 RNA consensus sequence
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additional information
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substrate specificity analysis by NMR binding and affinity study for RNA, overview. The enzyme is highly specific for pseudouridine. Methanocaldococcus jannaschii Nep1 binds to the yeast Nep1 RNA consensus sequence
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additional information
?
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substrate specificity analysis by NMR binding and affinity study for RNA, overview. The enzyme is highly specific for pseudouridine. Methanocaldococcus jannaschii Nep1 binds to the yeast Nep1 RNA consensus sequence
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