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2.1.1.256: tRNA (guanine6-N2)-methyltransferase

This is an abbreviated version!
For detailed information about tRNA (guanine6-N2)-methyltransferase, go to the full flat file.

Word Map on EC 2.1.1.256

Reaction

S-adenosyl-L-methionine
+
guanine6 in tRNA
=
S-adenosyl-L-homocysteine
+
N2-methylguanine6 in tRNA

Synonyms

G6 MTase, m2G6 methyltransferase, m2G6 tRNA methylase, methyltransferase Trm14, PF1002, PfTrm14, Trm14, TrmN, tRNA (m2G6) methyltransferase, tRNA m2G6 methyltransferase, tRNA m2G6 MTase, tRNA:m2G6 methyltransferase, TTC1157, TTCTrmN

ECTree

     2 Transferases
         2.1 Transferring one-carbon groups
             2.1.1 Methyltransferases
                2.1.1.256 tRNA (guanine6-N2)-methyltransferase

Crystallization

Crystallization on EC 2.1.1.256 - tRNA (guanine6-N2)-methyltransferase

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CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
purified recombinant detagged enzyme in complex with S-adenosyl-L-methionine or reaction product S-adenosyl-homocysteine, and inhibitor sinefungin, hanging drop vapor diffusion, protein in 50 mM Tris-HCl, pH 8.0, 10 mM MgCl2, 500 mM NaCl, 280 mM imidazole, and 1 mM DTT, mixing in a 1:1 ratio with crystallization solution containing 100 mM Tris-acetate, pH 8.0, 32% PEG 4000, and 15% glycerol, for the phasing, selenomethionine-derivatized PfTrm14 is crystallized in a crystallization solution containing 100 mM Tris-acetate, pH 8.0, 32% PEG 4000, 15% glycerol, after streak seeding from a native PfTrm14 crystal, crystal soaking in mother liquor containing 1 mM ligand, X-ray diffraction structure determination and analysis at 1.95-2.4 A resolution
purified recombinant His-tagged enzyme in complex with S-adenosyl-L-methionine or reaction product S-adenosyl-homocysteine, and inhibitor sinefungin, hanging drop vapor diffusion, protein in 50 mM Tris-HCl, pH 8.0, 250 mM NaCl, and 350 mM imidazole, mixing in a 1:1 ratio with crystallization solution 100 mM citrate/phosphate, pH 3.5, 15% PEG 6000, 200 mM NaCl, and 100 mM sodium citrate, crystal soaking in mother liquor containing 1 mM ligand, X-ray diffraction structure determination and analysis at 2.16 A resolution, solved in the apo state by molecular replacement to a resolution of 2.05 A