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2.1.1.247: [methyl-Co(III) methylamine-specific corrinoid protein]:coenzyme M methyltransferase

This is an abbreviated version!
For detailed information about [methyl-Co(III) methylamine-specific corrinoid protein]:coenzyme M methyltransferase, go to the full flat file.

Reaction

a [methyl-Co(III) methylamine-specific corrinoid protein]
+
CoM
=
methyl-CoM
+
a [Co(I) methylamine-specific corrinoid protein]

Synonyms

corrinoid:coenzyme M methyltransferase, methylated MtmC protein:CoM methyltransferase, methylated [methylamine-specific corrinoid protein]:coenzymeM methyltransferase, methylcobamide:coenzyme M methyltransferase, methylcobamide:CoM methyltransferase, methylcorrinoid:Coenzyme M methyltransferase, methylcorrinoid:CoM methyltransferase, methyltransferase 2, methyltransferase II, MMA-specific methyltransferase, MMA:CoM methyl transferase, MM_1070, MT2, MT2-A, MT2-M, mtaA, mtbA, TMA-specific methyltransferase, TMA:CoM methyl transferase, TMA:CoM methyltransferase, trimethylamine:coenzyme M methyltransferase

ECTree

     2 Transferases
         2.1 Transferring one-carbon groups
             2.1.1 Methyltransferases
                2.1.1.247 [methyl-Co(III) methylamine-specific corrinoid protein]:coenzyme M methyltransferase

Purification

Purification on EC 2.1.1.247 - [methyl-Co(III) methylamine-specific corrinoid protein]:coenzyme M methyltransferase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
native enzyme by two different steps of anion exchange chromatography, gel filtration, and another step of anion exchange chromatography, co-purification with the 26-kDa the trimethylamine-specific corrinoid protein
recombinant His6-tagged isozyme MT2-A from Escherichia coli strain M15 by anion exchange and hydrophobic interaction chromatography, and ultrafiltration to approximately 98% purity