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2.1.1.244: protein N-terminal methyltransferase

This is an abbreviated version!
For detailed information about protein N-terminal methyltransferase, go to the full flat file.

Word Map on EC 2.1.1.244

Reaction

2 S-adenosyl-L-methionine +

N-terminal-PPK-[protein]
= 2 S-adenosyl-L-homocysteine +
N-terminal-N,N-dimethyl-N-PPK-[protein]

Synonyms

alpha-N-methyltransferase, alpha-N-terminal methyltransferase 1, alphaN-methyltransferase, CG1675, dNTMT, EEF1A lysine methyltransferase 1, eEF1A-KMT1, EEF1AKMT1, Efm5, Efm7, elongation factor methyltransferase 7, FEAT, METT11B, METTL11A, METTL11a/C9orf32/Ad-003, METTL13, METTL13/FEAT, N-terminal and lysine methyltransferase, N-terminal methyltransferase, N-terminal methyltransferase 1, N-terminal RCC1 methyltransferase, N-terminal RCC1 methyltransferase 1, N-terminal Xaa-Pro-Lys N-methyltransferase 1, N6AMT2, NMT1, NNT1, NRMT, NRMT1, Ntm1, NTMT1, peptide N-terminal methyltransferase, PrmA, protein methyltransferase, protein N-terminal methyltransferase 1, Rkm2, SS alphaN-methyltransferase, SSMT, TAE1, YBR261C, YBR261C/Tae1, YGR001C, YLR285W

ECTree

     2 Transferases
         2.1 Transferring one-carbon groups
             2.1.1 Methyltransferases
                2.1.1.244 protein N-terminal methyltransferase

Crystallization

Crystallization on EC 2.1.1.244 - protein N-terminal methyltransferase

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CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
crystal structures with PDB IDs 3CJT, 3CJS, and 3CJR
analysis of crystal structures of NRMT1 and NRMT2 (PDB IDs 2EX4 and 5UBB, determined to 1.75 and 2.0 A, respectively), homology modeling
crystal structure with PDB IDs 5WCJ
crystal structures with PDB IDs 2EX4, 5E1B, 5E1M, 5E1O, 5E1D, 5E2B, 5E2A, 5CVD, and 5CVE
purified recombinant His-tagged enzyme in complex with inhibitor NAM-C3-PKRIA-NH2 , hanging drop vapor diffusion method, mixing of 30 mg/ml protein and 2 mM ligand, with 1.5 M lithium sulfate and 0.1 M Na-HEPES, pH 7.5, 20°C, X-ray diffraction structure determination and analysis at 1.47 A resolution, molecular replacement using the structure PDB ID 6DTN as template, and modelling
purified recombinant NRMT1 bound to CENP-A peptide and to the fruit fly H2B peptide in presence of S-adenosyl-L-homocysteine, vapor diffusion method, X-ray diffraction structure determination and analysis at 1.3 A and 1.5 A resolution, respectively
recombinant purified full-length wild-type NTMT1 in complex with S-adenosyl-L-homocysteine and a peptide derived from either human (SPKRIA) or mouse (PPKRIA) RCC1, or crystals of NTMT1 in complex with S-adenosyl-L-homocysteine and either the RPK or YPK substrate peptide, sitting drop vapor diffusion method, mixing of 0.001 ml of 37 mg/ml protein and ligand in 20 mM Tris-HCl, pH 7.5, 150 mM NaCl, and 0.5 mM TECP, with 0.001 ml of reservoir solution containing 23-28% PET 3350 and 14-18% Tacsimate, pH 6.0, X-ray diffraction structure determination and analysis, molecular replacement
-
crystal structures with PDB IDs 3EGV, 2NXC, and 2NXN