Any feedback?
Please rate this page
(all_enzymes.php)
(0/150)

BRENDA support

2.1.1.240: trans-resveratrol di-O-methyltransferase

This is an abbreviated version!
For detailed information about trans-resveratrol di-O-methyltransferase, go to the full flat file.

Word Map on EC 2.1.1.240

Reaction

2 S-adenosyl-L-methionine +

trans-resveratrol
= 2 S-adenosyl-L-homocysteine +
pterostilbene

Synonyms

5-pentadecatrienyl resorcinol O-methyltransferase, MT, resveratrol O-methyltransferase, ROMT, SbOMT3, SbROMT3syn, SbROMT3syn protein, VrROMT, VrROMTsyn, VrROMTsyn protein, VvRO

ECTree

     2 Transferases
         2.1 Transferring one-carbon groups
             2.1.1 Methyltransferases
                2.1.1.240 trans-resveratrol di-O-methyltransferase

Cloned

Cloned on EC 2.1.1.240 - trans-resveratrol di-O-methyltransferase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli. Transient coexpression of ROMT and grapevine stilbene synthase in tobacco (Nicotiana benthamiana) using the agroinfiltration technique results in the accumulation of pterostilbene in tobacco tissues
-
gene ROMT, quantitative RT-PCR enzyme expression analysis
gene ROMT, recombinant expression in Corynebacterium glutamicum and in Vitis vinifera cell culture, coexpression with flavanone 3-hydroxylase, and flavonol synthase, transient recombinant expression of HA-tagged enzyme in Nicotiana benthamiana leaves via Agrobacterium-mediated transient transformation, coexpression with human cytochrome P450 hydroxylase 1B1 (HsCYP1B1)
gene ROMT, recombinant expression in Escherichia coli strain BW27784 and in Saccharomyces cerevisiae, coexpression of two additional genes from the resveratrol biosynthetic pathway, 4-coumarate CoA-ligase and stilbene synthase, leads to production of a large amount of pterostilbene with a trace amount of pinostilbene, subcloning in Escherichia coli strain DH5alpha, recombinant expression of grape SUMO-fusion wild-type and mutant enzymes in Escherichia coli strain BL21 (DE3)
gene ROMT, recombinant expression of HA-tagged enzyme in Nicotiana tabacum strain SR1 via Agrobacterium tumefaciens strain LBA4404 transformation method, coexpression with genes RpSTS encoding stilbene synthase from Rheum palmatum cv. Jangyeop, real-time quantitative RT-PCR enzyme expression analysis
gene sbOMT3, recombinant expression of codon-optimized resveratrol O-methyltransferase genes sbOMT1 and sbOMT3 in Escherichia coli strain C41 (DE3), development of an artificial biosynthetic pathway that produces methylated resveratrol analogues, such as pinostilbene (3,4'-dihydroxy-5-methoxystilbene), 3,5-dihydroxy-4'-methoxystilbene, 3,4'-dimethoxy-5-hydroxystilbene, and 3,5,4'-trimethoxystilbene, from simple carbon sources in Escherichia coli
gene SbROMT3syn, recombinant expression of the His-tagged enzyme in Escherichia coli strain BL21-CodonPlus (DE3)-RIPL, subcloning in Escherichia coli strain DH5alpha
gene VrROMTsyn, recombinant expression of the His-tagged enzyme in Escherichia coli strain BL21-CodonPlus (DE3)-RIPL, subcloning in Escherichia coli strain DH5alpha. Recombinantly expressed enzyme from grape produces very small amounts of methylated resveratrol derivatives in Escherichia coli
recombinant expression in Escherichia coli strains BL21(DE3) and P1, coexpression with genes encoding Saccharothrix espanaensis TAL, Nicotiana tabacum CCL, and Vitis vinifera STS
-
recombinant expression of the His-tagged enzyme in Escherichia coli strain BL21-CodonPlus (DE3)-RIPL, coexpression with His-tagged cinnamate/4-coumarate:coenzyme A ligase from Streptomyces coelicolor and Strep-tagged stilbene synthase from Rheum palmatum
recombinant expression of the His-tagged enzyme in Escherichia coli strain BL21-CodonPlus (DE3)-RIPL, coexpression with His-tagged cinnamate/4-coumarate:coenzyme A ligase from Streptomyces coelicolor and Strep-tagged stilbene synthase from Rheum palmatum. Method evaluation and optimization