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2.1.1.233: [phosphatase 2A protein]-leucine-carboxy methyltransferase

This is an abbreviated version!
For detailed information about [phosphatase 2A protein]-leucine-carboxy methyltransferase, go to the full flat file.

Word Map on EC 2.1.1.233

Reaction

S-adenosyl-L-methionine
+
[phosphatase 2A protein]-leucine
=
S-adenosyl-L-homocysteine
+
[phosphatase 2A protein]-leucine methyl ester

Synonyms

C-terminal leucine protein carboxyl methyltransferase-1, LCMT-1, LCMT1, leucine carboxy methyltransferase-1, leucine carboxyl methyl transferase 1, leucine carboxyl methyltransferase, leucine carboxyl methyltransferase 1, leucine carboxyl methyltransferase 1 homolog, leucine carboxyl methyltransferase-1, leucine carboxylmethyltransferase-1, leucine carboxymethyltransferase 1/phosphatase methylesterase 1, phosphatase 2A protein methyltransferase, PME-1, PP2A LCMT, PP2A methyltransferase, PP2A-methyltransferase, PP2A-specific methyltransferase, PPM1, Ppm1p, PPMT, protein phosphatase 2A leucine carboxyl methyltransferase, protein phosphatase methyltransferase 1

ECTree

     2 Transferases
         2.1 Transferring one-carbon groups
             2.1.1 Methyltransferases
                2.1.1.233 [phosphatase 2A protein]-leucine-carboxy methyltransferase

Crystallization

Crystallization on EC 2.1.1.233 - [phosphatase 2A protein]-leucine-carboxy methyltransferase

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CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
enzyme in isolation and in complex with phosphatase 2A protein stabilized by a cofactor mimic, hanging drop vapor diffusion method, using 17-19% (w/v) PEG2000 monomethyl ether, 150 mM triethylamine N-oxide, and 5 mM dithiothreitol
-
in complex with the cofactor S-adenosylmethionine, hanging drop vapor diffusion method
-
native protein alone or in complexes with the S-adenosyl-L-methionine methyl donor and the S-adenosyl-L-homocysteine product and inhibitor, hanging drop vapor diffusion method, using 15% (w/v) PEG 8000 and 0.1 M potassium or sodium phosphate, pH 4.6, or 20% (w/v) PEG 8000, 0.1 M sodium acetate, and 0.1 M MES, pH 5.6, or 24% (w/v) PEG 4000, 0.2 M magnesium chloride, and 0.1 M Tris-HCl, pH 8.5