2.1.1.229: tRNA (carboxymethyluridine34-5-O)-methyltransferase
This is an abbreviated version!
For detailed information about tRNA (carboxymethyluridine34-5-O)-methyltransferase, go to the full flat file.
Word Map on EC 2.1.1.229
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2.1.1.229
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wobble
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methyltransferases
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5-methoxycarbonylmethyluridine
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ribonucleotide
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anticodon
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gene-specific
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trnagluuuc
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translationally
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medicine
- 2.1.1.229
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wobble
- methyltransferases
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5-methoxycarbonylmethyluridine
- ribonucleotide
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anticodon
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gene-specific
- trnagluuuc
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translationally
- medicine
Reaction
Synonyms
ABH8, ALKBH8, CmoA, MnmC, Trm9, Trm9-Trm112, Trm9p, TrmC, tRNA methyltransferase 9, YfcK, YML014w
ECTree
2.1.1.229 tRNA (carboxymethyluridine34-5-O)-methyltransferaseAdvanced search results
results (
results (Substrates Products
Substrates Products on EC 2.1.1.229 - tRNA (carboxymethyluridine34-5-O)-methyltransferase
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SUBSTRATE 
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + carboxymethyl-2-thiouridine34 in tRNA
S-adenosyl-L-homocysteine + 5-(2-methoxy-2-oxoethyl)-2-thiouridine34 in tRNA
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Trm9-Trm112, subunit Trm112 is required for the activity, but role of Sc Trm112 in the complex is not for catalysis
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-
?
S-adenosyl-L-methionine + carboxymethylaminomethyl 2-thiouridine34 in tRNAGlu
S-adenosyl-L-homocysteine + methylaminomethyl 2-thiouridine34 in tRNAGlu + hydroxyacetate
S-adenosyl-L-methionine + carboxymethylaminomethyl 2-thiouridine34 in tRNALys
S-adenosyl-L-homocysteine + methylaminomethyl 2-thiouridine34 in tRNALys + hydroxyacetate
S-adenosyl-L-methionine + carboxymethylaminomethyl uridine34 in tRNAArg
S-adenosyl-L-homocysteine + methylaminomethyl uridine34 in tRNAArg + hydroxyacetate
S-adenosyl-L-methionine + carboxymethyluridine34 in tRNA
S-adenosyl-L-homocysteine + 5-(2-methoxy-2-oxoethyl)uridine34 in tRNA
S-adenosyl-L-methionine + carboxymethyluridine34 in tRNAArg3
S-adenosyl-L-homocysteine + 5-(2-methoxy-2-oxoethyl)uridine34 in tRNA
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-
-
?
S-adenosyl-L-methionine + carboxymethyluridine34 in tRNAGlU
S-adenosyl-L-homocysteine + 5-(2-methoxy-2-oxoethyl)uridine34 in tRNA
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-
-
?
S-adenosyl-S-carboxymethyl-L-homocysteine + 5-methoxyuridine34 in tRNA
S-adenosyl-L-methionine + uridine 5-oxyacetic acid in tRNA
proposed modification pathway of 5-oxyuridine derivatives, overview
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-
?
S-adenosyl-L-methionine + carboxymethylaminomethyl 2-thiouridine34 in tRNAGlu
S-adenosyl-L-homocysteine + methylaminomethyl 2-thiouridine34 in tRNAGlu + hydroxyacetate
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-
-
-
?
S-adenosyl-L-methionine + carboxymethylaminomethyl 2-thiouridine34 in tRNAGlu
S-adenosyl-L-homocysteine + methylaminomethyl 2-thiouridine34 in tRNAGlu + hydroxyacetate
-
-
-
?
S-adenosyl-L-methionine + carboxymethylaminomethyl 2-thiouridine34 in tRNAGlu
S-adenosyl-L-homocysteine + methylaminomethyl 2-thiouridine34 in tRNAGlu + hydroxyacetate
-
-
-
?
S-adenosyl-L-methionine + carboxymethylaminomethyl 2-thiouridine34 in tRNALys
S-adenosyl-L-homocysteine + methylaminomethyl 2-thiouridine34 in tRNALys + hydroxyacetate
-
-
-
-
?
S-adenosyl-L-methionine + carboxymethylaminomethyl 2-thiouridine34 in tRNALys
S-adenosyl-L-homocysteine + methylaminomethyl 2-thiouridine34 in tRNALys + hydroxyacetate
-
-
-
?
S-adenosyl-L-methionine + carboxymethylaminomethyl 2-thiouridine34 in tRNALys
S-adenosyl-L-homocysteine + methylaminomethyl 2-thiouridine34 in tRNALys + hydroxyacetate
-
-
-
?
S-adenosyl-L-methionine + carboxymethylaminomethyl uridine34 in tRNAArg
S-adenosyl-L-homocysteine + methylaminomethyl uridine34 in tRNAArg + hydroxyacetate
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-
-
-
?
S-adenosyl-L-methionine + carboxymethylaminomethyl uridine34 in tRNAArg
S-adenosyl-L-homocysteine + methylaminomethyl uridine34 in tRNAArg + hydroxyacetate
-
-
-
?
S-adenosyl-L-methionine + carboxymethylaminomethyl uridine34 in tRNAArg
S-adenosyl-L-homocysteine + methylaminomethyl uridine34 in tRNAArg + hydroxyacetate
-
-
-
?
S-adenosyl-L-methionine + carboxymethyluridine34 in tRNA
S-adenosyl-L-homocysteine + 5-(2-methoxy-2-oxoethyl)uridine34 in tRNA
-
-
-
?
S-adenosyl-L-methionine + carboxymethyluridine34 in tRNA
S-adenosyl-L-homocysteine + 5-(2-methoxy-2-oxoethyl)uridine34 in tRNA
residue Arg567 os involved in tRNA substrate recognition
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-
?
S-adenosyl-L-methionine + carboxymethyluridine34 in tRNA
S-adenosyl-L-homocysteine + 5-(2-methoxy-2-oxoethyl)uridine34 in tRNA
the enzyme catalyzes tRNA methylation to generate 5-methylcarboxymethyl uridine at the wobble position of certain tRNAs, a critical anticodon loop modification linked to DNA damage survival
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-
?
S-adenosyl-L-methionine + carboxymethyluridine34 in tRNA
S-adenosyl-L-homocysteine + 5-(2-methoxy-2-oxoethyl)uridine34 in tRNA
ABH8 catalyzes RNA methylation through its methyltransferase domain. ABH8 MT domain alone exhibited weak methylase activity
5-(2-methoxy-2-oxoethyl)uridine i.e. 5-methoxycarboxymethyluridine
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?
S-adenosyl-L-methionine + carboxymethyluridine34 in tRNA
S-adenosyl-L-homocysteine + 5-(2-methoxy-2-oxoethyl)uridine34 in tRNA
-
-
-
-
?
S-adenosyl-L-methionine + carboxymethyluridine34 in tRNA
S-adenosyl-L-homocysteine + 5-(2-methoxy-2-oxoethyl)uridine34 in tRNA
Trm9 methylates the uridine wobble base of tRNAArg(UCU) and tRNAGlU(UUC)
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-
?
S-adenosyl-L-methionine + carboxymethyluridine34 in tRNA
S-adenosyl-L-homocysteine + 5-(2-methoxy-2-oxoethyl)uridine34 in tRNA
Trm9 methylates the uridine wobble base of tRNAGlU(UUC)
-
-
?
S-adenosyl-L-methionine + carboxymethyluridine34 in tRNA
S-adenosyl-L-homocysteine + 5-(2-methoxy-2-oxoethyl)uridine34 in tRNA
-
Trm9-Trm112, subunit Trm112 is required for the activity, but role of Sc Trm112 in the complex is not for catalysis
-
-
?
additional information
?
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the bifunctional enzyme MnmC catalyzes the two consecutive reactions that convert 5-carboxymethylaminomethyl uridine to 5-methylaminomethyl uridine. The C-terminal domain of MnmC is responsible for the FAD-dependent deacetylation of cmnm5U to 5-aminomethyl uridine, whereas the N-terminal domain catalyzes the subsequent S-adenosyl-L-methionine-dependent methylation of 5-aminomethyl uridine, leading to the final product, 5-methylaminomethyl uridine, coordination of the two consecutive reactions, overview
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-
?
additional information
?
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methylaminomethyl modification of uridine or 2-thiouridine (mnm5U34 or mnm5s2U34) at the wobble position of tRNAs specific for glutamate, lysine and arginine are observed in Escherichia coli and allow for specific recognition of codons ending in A or G. In the biosynthetic pathway responsible for this posttranscriptional modification, the bifunctional enzyme MnmC catalyzes the conversion of its hypermodified substrate carboxymethylaminomethyl uridine (cmnm5U34) to mnm5U34. MnmC catalyzes the FAD-dependent oxidative cleavage of carboxymethyl group from cmnm5U34 via an imine intermediate to generate aminomethyl uridine (nm5U34), which is subsequently methylated by S-adenosyl-L-methionine to yield methylaminomethyl uridine (mnm5U34)
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?
additional information
?
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alternative mechanisms in formation of the 5-carboxymethyluridine34 side chain at wobble position, overview
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-
?
additional information
?
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recombinant tagged Sc Trm9 protein purified from yeast methylates a saponified tRNA extract, demonstrating that Sc Trm9 is required for formation of the terminal methyl group of mcm5U
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-
?
additional information
?
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methylaminomethyl modification of uridine or 2-thiouridine (mnm5U34 or mnm5s2U34) at the wobble position of tRNAs specific for glutamate, lysine and arginine are observed in Escherichia coli and allow for specific recognition of codons ending in A or G. In the biosynthetic pathway responsible for this posttranscriptional modification, the bifunctional enzyme MnmC catalyzes the conversion of its hypermodified substrate carboxymethylaminomethyl uridine (cmnm5U34) to mnm5U34. MnmC catalyzes the FAD-dependent oxidative cleavage of carboxymethyl group from cmnm5U34 via an imine intermediate to generate aminomethyl uridine (nm5U34), which is subsequently methylated by S-adenosyl-L-methionine to yield methylaminomethyl uridine (mnm5U34)
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-
?
additional information
?
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methylaminomethyl modification of uridine or 2-thiouridine (mnm5U34 or mnm5s2U34) at the wobble position of tRNAs specific for glutamate, lysine and arginine are observed in Escherichia coli and allow for specific recognition of codons ending in A or G. In the biosynthetic pathway responsible for this posttranscriptional modification, the bifunctional enzyme MnmC catalyzes the conversion of its hypermodified substrate carboxymethylaminomethyl uridine (cmnm5U34) to mnm5U34. MnmC catalyzes the FAD-dependent oxidative cleavage of carboxymethyl group from cmnm5U34 via an imine intermediate to generate aminomethyl uridine (nm5U34), which is subsequently methylated by S-adenosyl-L-methionine to yield methylaminomethyl uridine (mnm5U34)
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-
?
