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2.1.1.229: tRNA (carboxymethyluridine34-5-O)-methyltransferase

This is an abbreviated version!
For detailed information about tRNA (carboxymethyluridine34-5-O)-methyltransferase, go to the full flat file.

Word Map on EC 2.1.1.229

Reaction

S-adenosyl-L-methionine
+
carboxymethyluridine34 in tRNA
=
S-adenosyl-L-homocysteine
 +
5-(2-methoxy-2-oxoethyl)uridine34 in tRNA

Synonyms

ABH8, ALKBH8, CmoA, MnmC, Trm9, Trm9-Trm112, Trm9p, TrmC, tRNA methyltransferase 9, YfcK, YML014w

ECTree

     2 Transferases
         2.1 Transferring one-carbon groups
             2.1.1 Methyltransferases
                2.1.1.229 tRNA (carboxymethyluridine34-5-O)-methyltransferase

Cloned

Cloned on EC 2.1.1.229 - tRNA (carboxymethyluridine34-5-O)-methyltransferase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
co-expression of His-tagged Trm9p with untagged Trm112p in Escherichia coli strain BL21(DE3)pLysS, Trm9p/Trm112p complex formation
-
expression in Escherichia coli
expression of GST-Trm9 fusion protein in Escherichia coli DH5alpha cells
gene cmoA, recombinant expression of N-terminally His-tagged enzyme in Escherichia coli strain Rosetta pLysS (DE3)
gene JW5380, expression of wild-type and selenomethinone-labeled MnmC in Escherichia coli strains Rosetta2(DE3) and B834(DE3), respectively
gene mnmC, recombinant expression of N-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3)
-
gene mnmC, recombinant expression of N-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3), expression of selenomethionine-labeled enzyme in Escherichia coli strain B834
the FLAG-tagged-ABH8 coding region is cloned into the pBABEpuro or pBABEhygro retroviral vector. HeLa S3 cells are infected with retrovirus