2.1.1.224: 23S rRNA (adenine2503-C8)-methyltransferase

This is an abbreviated version!
For detailed information about 23S rRNA (adenine2503-C8)-methyltransferase, go to the full flat file.

Reaction

2 S-adenosyl-L-methionine +

Synonyms

antibiotic resistance protein, Ba Cfr, C8 adenine RNA methylase, Cd Cfr, Cfr, Cfr methyltransferase, Cfr rRNA methyltransferase, cfr(C), Cfr(E), cfr-like, Cfr-like protein, ClbA, ClbB, ClbC, EC 2.1.1.194, Ef Cfr, Pl Cfr, radical AdoMet rRNA methyltransferase, radical S-adenosylmethionine enzyme, radical S-adenosylmethionine methylase, radical-S-adenosyl-L-methionine enzyme, radical-SAM enzyme, radical-SAM rRNA methyltransferase, RS methylase

ECTree

     2 Transferases

         2.1 Transferring one-carbon groups

             2.1.1 Methyltransferases

                2.1.1.224 23S rRNA (adenine2503-C8)-methyltransferase

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Results	in table
1	Activating Compound
245	AA Sequence
1	CAS Registry Number
21	Cloned(Commentary)
36	Cofactor
1	Crystallization (Commentary)
2	Expression
66	General Information
5	KM Value [mM]
10	Metals/Ions
3	Molecular Weight [Da]
35	Natural Substrates/ Products (Substrates)
65	Organism
1	Pathway
7	pH Optimum
28	Protein Variants
3	Purification (Commentary)
6	Reaction
41	Reference
2	Source Tissue
60	Substrates and Products (Substrate)
4	Subunits
82	Synonyms
1	Systematic Name
7	Temperature Optimum [°C]

Natural Substrates Products

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Natural Substrates Products on EC 2.1.1.224 - 23S rRNA (adenine2503-C8)-methyltransferase

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NATURAL SUBSTRATE

NATURAL PRODUCT

REACTION DIAGRAM

ORGANISM

UNIPROT

COMMENTARY
(Substrate)

LITERATURE
(Substrate)

COMMENTARY
(Product)

LITERATURE
(Product)

REVERSIBILITY
r=reversible
ir=irreversible
?=not specified

2 S-adenosyl-L-methionine + adenine2503 in 23S rRNA

S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 8-methyladenine2503 in 23S rRNA

9 entries

2 S-adenosyl-L-methionine + adenine2503 in 23S rRNA + 2 reduced [2Fe-2S] ferredoxin

S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 8-methyladenine2503 in 23S rRNA + 2 oxidized [2Fe-2S] ferredoxin

17 entries

2 S-adenosyl-L-methionine + adenine2503 in 23S rRNA + 2 reduced [4Fe-4S] ferredoxin

S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 8-methyladenine2503 in 23S rRNA + 2 oxidized [4Fe-4S] ferredoxin

4 entries

S-adenosyl-L-methionine + adenine2503 in 23S rRNA + reduced [2Fe-2S] ferredoxin

S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 8-methyladenine2503 in 23S rRNA + oxidized [2Fe-2S] ferredoxin

Mammaliicoccus sciuri

Q9FBG4

-

735095

-

-

?

additional information

?

-

3 entries

2 S-adenosyl-L-methionine + adenine2503 in 23S rRNA

S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 8-methyladenine2503 in 23S rRNA

Alkalihalobacillus clausii

Q5WJ42

-

718601

-

-

?

2 S-adenosyl-L-methionine + adenine2503 in 23S rRNA

S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 8-methyladenine2503 in 23S rRNA

Azotobacter vinelandii

-

the enzyme methylates the 8 position of 23S rRNA residue A2503 to confer resistance to multiple antibiotic classes acting upon the large subunit of the bacterial ribosome

720968

-

-

?

2 S-adenosyl-L-methionine + adenine2503 in 23S rRNA

S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 8-methyladenine2503 in 23S rRNA

Bacillus amyloliquefaciens

-

-

718601

-

-

?

2 S-adenosyl-L-methionine + adenine2503 in 23S rRNA

S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 8-methyladenine2503 in 23S rRNA

Bacillus amyloliquefaciens FZB42

-

-

718601

-

-

?

2 S-adenosyl-L-methionine + adenine2503 in 23S rRNA

S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 8-methyladenine2503 in 23S rRNA

Brevibacillus brevis

C0ZJA6

-

718601

-

-

?

2 S-adenosyl-L-methionine + adenine2503 in 23S rRNA

S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 8-methyladenine2503 in 23S rRNA

Brevibacillus brevis NBRC 100599

C0ZJA6

-

718601

-

-

?

2 S-adenosyl-L-methionine + adenine2503 in 23S rRNA

S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 8-methyladenine2503 in 23S rRNA

Escherichia coli

-

Cfr is an plasmid-acquired methyltransferase that protects cells from the action of antibiotics

704203

-

-

?

2 S-adenosyl-L-methionine + adenine2503 in 23S rRNA

S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 8-methyladenine2503 in 23S rRNA

Staphylococcus aureus

-

-

718587

-

-

?

2 S-adenosyl-L-methionine + adenine2503 in 23S rRNA

S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 8-methyladenine2503 in 23S rRNA

Staphylococcus aureus RN4220

-

-

718587

-

-

?

2 S-adenosyl-L-methionine + adenine2503 in 23S rRNA + 2 reduced [2Fe-2S] ferredoxin

S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 8-methyladenine2503 in 23S rRNA + 2 oxidized [2Fe-2S] ferredoxin

Bacillus amyloliquefaciens

A0A4V7TJX0

-

758141

-

-

?

2 S-adenosyl-L-methionine + adenine2503 in 23S rRNA + 2 reduced [2Fe-2S] ferredoxin

S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 8-methyladenine2503 in 23S rRNA + 2 oxidized [2Fe-2S] ferredoxin

Bacillus amyloliquefaciens DSM-7

A0A4V7TJX0

-

758141

-

-

?

2 S-adenosyl-L-methionine + adenine2503 in 23S rRNA + 2 reduced [2Fe-2S] ferredoxin

S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 8-methyladenine2503 in 23S rRNA + 2 oxidized [2Fe-2S] ferredoxin

Clostridioides difficile

-

-

755810

-

-

?

2 S-adenosyl-L-methionine + adenine2503 in 23S rRNA + 2 reduced [2Fe-2S] ferredoxin

S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 8-methyladenine2503 in 23S rRNA + 2 oxidized [2Fe-2S] ferredoxin

Clostridioides difficile

V5ZEQ2

-

756922, 758141

-

-

?

2 S-adenosyl-L-methionine + adenine2503 in 23S rRNA + 2 reduced [2Fe-2S] ferredoxin

S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 8-methyladenine2503 in 23S rRNA + 2 oxidized [2Fe-2S] ferredoxin

Clostridioides difficile DF11

-

-

755810

-

-

?

2 S-adenosyl-L-methionine + adenine2503 in 23S rRNA + 2 reduced [2Fe-2S] ferredoxin

S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 8-methyladenine2503 in 23S rRNA + 2 oxidized [2Fe-2S] ferredoxin

Clostridioides difficile QCD63q42

V5ZEQ2

-

758141

-

-

?

2 S-adenosyl-L-methionine + adenine2503 in 23S rRNA + 2 reduced [2Fe-2S] ferredoxin

S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 8-methyladenine2503 in 23S rRNA + 2 oxidized [2Fe-2S] ferredoxin

Clostridioides difficile T10

V5ZEQ2

-

756922

-

-

?

2 S-adenosyl-L-methionine + adenine2503 in 23S rRNA + 2 reduced [2Fe-2S] ferredoxin

S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 8-methyladenine2503 in 23S rRNA + 2 oxidized [2Fe-2S] ferredoxin

Enterocloster bolteae

-

-

756922

-

-

?

2 S-adenosyl-L-methionine + adenine2503 in 23S rRNA + 2 reduced [2Fe-2S] ferredoxin

S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 8-methyladenine2503 in 23S rRNA + 2 oxidized [2Fe-2S] ferredoxin

Enterocloster bolteae 90B3

-

-

756922

-

-

?

2 S-adenosyl-L-methionine + adenine2503 in 23S rRNA + 2 reduced [2Fe-2S] ferredoxin

S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 8-methyladenine2503 in 23S rRNA + 2 oxidized [2Fe-2S] ferredoxin

Enterococcus faecalis

A0A2Z6BLR4

-

758141

-

-

?

2 S-adenosyl-L-methionine + adenine2503 in 23S rRNA + 2 reduced [2Fe-2S] ferredoxin

S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 8-methyladenine2503 in 23S rRNA + 2 oxidized [2Fe-2S] ferredoxin

Enterococcus faecalis TX0645

A0A2Z6BLR4

-

758141

-

-

?

2 S-adenosyl-L-methionine + adenine2503 in 23S rRNA + 2 reduced [2Fe-2S] ferredoxin

S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 8-methyladenine2503 in 23S rRNA + 2 oxidized [2Fe-2S] ferredoxin

Escherichia coli

-

-

758463

-

-

?

2 S-adenosyl-L-methionine + adenine2503 in 23S rRNA + 2 reduced [2Fe-2S] ferredoxin

S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 8-methyladenine2503 in 23S rRNA + 2 oxidized [2Fe-2S] ferredoxin

Mammaliicoccus sciuri

Q9FBG4

-

758128

-

-

?

2 S-adenosyl-L-methionine + adenine2503 in 23S rRNA + 2 reduced [2Fe-2S] ferredoxin

S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 8-methyladenine2503 in 23S rRNA + 2 oxidized [2Fe-2S] ferredoxin

Paenibacillus lautus

D3EIG6

-

758141

-

-

?

2 S-adenosyl-L-methionine + adenine2503 in 23S rRNA + 2 reduced [2Fe-2S] ferredoxin

S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 8-methyladenine2503 in 23S rRNA + 2 oxidized [2Fe-2S] ferredoxin

Paenibacillus lautus Y412MC10

D3EIG6

-

758141

-

-

?

2 S-adenosyl-L-methionine + adenine2503 in 23S rRNA + 2 reduced [2Fe-2S] ferredoxin

S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 8-methyladenine2503 in 23S rRNA + 2 oxidized [2Fe-2S] ferredoxin

Staphylococcus aureus

A5HBL2

-

758461

-

-

?

2 S-adenosyl-L-methionine + adenine2503 in 23S rRNA + 2 reduced [2Fe-2S] ferredoxin

S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 8-methyladenine2503 in 23S rRNA + 2 oxidized [2Fe-2S] ferredoxin

Staphylococcus sp.

-

-

755805

-

-

?

2 S-adenosyl-L-methionine + adenine2503 in 23S rRNA + 2 reduced [4Fe-4S] ferredoxin

S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 8-methyladenine2503 in 23S rRNA + 2 oxidized [4Fe-4S] ferredoxin

Clostridioides difficile

V5ZEZ1

-

735450

-

-

?

2 S-adenosyl-L-methionine + adenine2503 in 23S rRNA + 2 reduced [4Fe-4S] ferredoxin

S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 8-methyladenine2503 in 23S rRNA + 2 oxidized [4Fe-4S] ferredoxin

Mammaliicoccus sciuri

Q9FBG4

-

733111

-

-

?

2 S-adenosyl-L-methionine + adenine2503 in 23S rRNA + 2 reduced [4Fe-4S] ferredoxin

S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 8-methyladenine2503 in 23S rRNA + 2 oxidized [4Fe-4S] ferredoxin

Staphylococcus aureus

-

-

735095

-

-

?

2 S-adenosyl-L-methionine + adenine2503 in 23S rRNA + 2 reduced [4Fe-4S] ferredoxin

S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 8-methyladenine2503 in 23S rRNA + 2 oxidized [4Fe-4S] ferredoxin

Staphylococcus aureus

A5HBL2

-

736280

-

-

?

additional information

?

-

Escherichia coli K-12

P36979

Cfr confers a phenotype with resistance to phenicols, lincosamides, oxazolidinones, pleuromutilins, and streptogramin A antibiotics. Methylation of position 8 is the dominant antibiotic resistance determinant, but indigenous modification at position 2 also contributes to low-level resistance

706726

-

-

?

additional information

?

-

Staphylococcus aureus

-

enzyme Cfr methylates adenosine 2503 of the 23S rRNA in the peptidyltransferase centre (P-site) of the bacterial ribosome. In wild-type Cfr, where Cys338 is methylated, S-adenosyl-L-methionine binding leads to rapid oxidation of the [4Fe-4S] cluster and production of 5'-deoxyadenosine

735095

-

-

?

additional information

?

-

Staphylococcus aureus

A5HBL2

methylation at C8 is similar to that at C2, cf. EC 2.1.1.192

736280

-

-

?