2.1.1.224: 23S rRNA (adenine2503-C8)-methyltransferase
This is an abbreviated version!
For detailed information about 23S rRNA (adenine2503-C8)-methyltransferase, go to the full flat file.
Word Map on EC 2.1.1.224
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2.1.1.224
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linezolid
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oxazolidinone
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5\'-deoxyadenosyl
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thiopeptide
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optra
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linezolid-resistant
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phenicols
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cobalamin-dependent
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phlopsa
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pleuromutilins
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nosiheptide
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5'-deoxyadenosine
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poxta
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ribosome-targeting
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cobiialamin
- 2.1.1.224
- linezolid
-
oxazolidinone
-
5\'-deoxyadenosyl
-
thiopeptide
-
optra
-
linezolid-resistant
-
phenicols
-
cobalamin-dependent
-
phlopsa
-
pleuromutilins
-
nosiheptide
- 5'-deoxyadenosine
-
poxta
-
ribosome-targeting
-
cobiialamin
Reaction
2 S-adenosyl-L-methionine
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Synonyms
antibiotic resistance protein, Ba Cfr, C8 adenine RNA methylase, Cd Cfr, Cfr, Cfr methyltransferase, Cfr rRNA methyltransferase, cfr(C), Cfr(E), cfr-like, Cfr-like protein, ClbA, ClbB, ClbC, EC 2.1.1.194, Ef Cfr, Pl Cfr, radical AdoMet rRNA methyltransferase, radical S-adenosylmethionine enzyme, radical S-adenosylmethionine methylase, radical-S-adenosyl-L-methionine enzyme, radical-SAM enzyme, radical-SAM rRNA methyltransferase, RS methylase
ECTree
2.1.1.224 23S rRNA (adenine2503-C8)-methyltransferaseAdvanced search results
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results (Engineering
Engineering on EC 2.1.1.224 - 23S rRNA (adenine2503-C8)-methyltransferase
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PROTEIN VARIANTS 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
C112A
C116A
C119A
R25A
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mutation lowers the resistance to both florfenicol and tiamulin considerably
S189A
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mutation lowers the resistance to both florfenicol and tiamulin considerably
C115A
mutation of the cysteines in the presumed radical S-adenosyl-L-methionine motif CxxxCxxC abolishes Cfr activity
C119A
mutation of the cysteines in the presumed radical S-adenosyl-L-methionine motif CxxxCxxC abolishes Cfr activity
C122A
mutation of the cysteines in the presumed radical S-adenosyl-L-methionine motif CxxxCxxC abolishes Cfr activity
C338A
the mutant binds S-adenosyl-L-methionine with wild type affinity, while oxidation of the [4Fe-4S] cluster is not observed
C119A
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site-directed mutagenesis, the mutant Cfr is inactive, and mutant cells show no resistance against antibiotics
C338A
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site-directed mutagenesis, Cys338Ala Cfr binds S-adenosyl-L-methionine with equivalent affinity, oxidation of the [4Fe-4S] cluster is not observed
C119A
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site-directed mutagenesis, the mutant Cfr is inactive, and mutant cells show no resistance against antibiotics
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additional information
additional information
construction of chimera between Cfr and RlmN from Echerichia coli to analyze C2/C8 and C2 methylation specificity, respectively. The catalytic site is expected to be responsible for the C2/C8 specificity. Almost all replacements show no function in the primer extension assay, apart from a few that have a weak effect
additional information
construction of chimeric mutants of gene cfr, cfr1234567rrlmN and rlmN1234567rcfr, and construction of mixed genes containing gene fragment of cfr and rlmN genes, overview. Analysis of effects of the mutations on catalytic activity and antibiotics resistance
additional information
insertion of the cfr region into the CM05 chromosome occurs at a beta/six site I recognition site. Identification of a subpopulation of cfr-negative CM05, CM05DELTA, arising from recombination between duplicate ermB regions, overview
additional information
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insertion of the cfr region into the CM05 chromosome occurs at a beta/six site I recognition site. Identification of a subpopulation of cfr-negative CM05, CM05DELTA, arising from recombination between duplicate ermB regions, overview
additional information
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insertion of the cfr region into the CM05 chromosome occurs at a beta/six site I recognition site. Identification of a subpopulation of cfr-negative CM05, CM05DELTA, arising from recombination between duplicate ermB regions, overview
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