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2.1.1.224: 23S rRNA (adenine2503-C8)-methyltransferase

This is an abbreviated version!
For detailed information about 23S rRNA (adenine2503-C8)-methyltransferase, go to the full flat file.

Word Map on EC 2.1.1.224

Reaction

2 S-adenosyl-L-methionine +

adenine2503 in 23S rRNA
+ 2 reduced [2Fe-2S] ferredoxin =
S-adenosyl-L-homocysteine
 +
L-methionine
 +
5'-deoxyadenosine
 +
8-methyladenine2503 in 23S rRNA
 + 2 oxidized [2Fe-2S] ferredoxin

Synonyms

antibiotic resistance protein, Ba Cfr, C8 adenine RNA methylase, Cd Cfr, Cfr, Cfr methyltransferase, Cfr rRNA methyltransferase, cfr(C), Cfr(E), cfr-like, Cfr-like protein, ClbA, ClbB, ClbC, EC 2.1.1.194, Ef Cfr, Pl Cfr, radical AdoMet rRNA methyltransferase, radical S-adenosylmethionine enzyme, radical S-adenosylmethionine methylase, radical-S-adenosyl-L-methionine enzyme, radical-SAM enzyme, radical-SAM rRNA methyltransferase, RS methylase

ECTree

     2 Transferases
         2.1 Transferring one-carbon groups
             2.1.1 Methyltransferases
                2.1.1.224 23S rRNA (adenine2503-C8)-methyltransferase

Cloned

Cloned on EC 2.1.1.224 - 23S rRNA (adenine2503-C8)-methyltransferase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
a plasmid-borne cfr geneis transfromed into a uL3-depleted Escherichia coli strain containing either wild-type L3 or L3 with one of seven mutations, G147R, Q148F, N149S, N149D, N149R, Q150L, or T151P, expressed from plasmid-carried rplC genes. The L3 mutations are well tolerated, with small to moderate growth rate decreases. The presence of Cfr has a very minor influence on the growth rate. The transformants show resistance to linezolid, tiamulin, florfenicol, and Synercid (a combination of quinupristin and dalfopristin [Q-D])
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expressed as C-terminally His6-tagged enzyme, wild-type and mutant enzymes C125A, C129A and C132A
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expressed in Escherichia coli Rosetta2(DE3)pLysS cells
gene cfr(C), DNA and amino acid sequence determination and analysis, quantitative RT-PCR expression analysis, cfr(C) is constitutively transcribed, gene cfr(C) is part of a putative 24 kb-transposon, which generates a detectable circular intermediate
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gene cfr(C), DNA and amino acid sequence determination and analysis, the cfr-like is cloned in Clostridium difficile strain 630DELTAerm in which it confers resistance to phenicols, lincosamides, oxazolidinones, pleuromutilins, and streptogramin A (PhLOPSA)
gene cfr(E), DNA and amino acid sequence determination and analysis, genotyping, sequence comparisons and phylogenetic analysis of different cfr gene variants, recombinant expression of tagged enzyme Cfr(E) in Escherichia coli strain Rosetta(DE3)pLysS
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gene cfr, operon isc, co-expression with isc proteins, expression of His6-tagged Cfr under control of an arabinose-inducible promoter in Escherichia coli strain BL21Star
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gene cfr, recombinant expression of N-terminally His6-tagged enzyme in Escherichia coli strain +Rosetta2(DE3)
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gene cfr, recombinant expression of wild-type and mutant enzymes in Escherichia coli strain JW2501-1 (rlmN knockout) and AS19
gene cfr, sequence comparisons and phylogenetic analysis and tree, overview
gene cfr, sequence comparisons and phylogenetic analysis, primer extension analysis, cloning and expression in Escherichia coli strains TOP10 and AS19
gene cfr, The Cfr-like protein in Peptoclostridium difficile is hosted by a transposon, and the same gene is found in other strains, sequence comparisons, overview. Recombinant expression in Escherichia coli strain AS19 which then shows elevated MIC values for five classes of antibiotics, and recombinant expression in rlmN-deficient Escherichia coli strain JW2505-1
gene cfr, while cfr is typically plasmid borne, in CM05 it is located on the chromosome and is coexpressed with ermB as part of the mlr operon. DNA and amino acid sequence determination and analysis and genetic organization, overview. The cfr-containing mlr operon is located within a 15.5-kb plasmid-like insertion into 23S rRNA allele 4. In the LZDs colonies, designated CM05DELTA, a recombination event involving the two ermB genes had occurred, resulting in the deletion of cfr and the 3= flanking region, cfr-istAS-istBS-ermB
gene clbA, DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression in Escherichia coli strain AS19 using plasmid pLJ10
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gene clbB, DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression in Escherichia coli strain AS19 using plasmid pLJ10
gene clbC, DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression in Escherichia coli strain AS19 using plasmid pLJ10
gene gfr, ermB and cfr genes are naturally coexpressed under the Perm promoter in the mlr operon, expression of mlr results in modification of A2058 and A2503 in 23S rRNA and renders cells resistant to all clinically relevant antibiotics that target the large ribosomal subunit
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