2.1.1.220: tRNA (adenine58-N1)-methyltransferase
This is an abbreviated version!
For detailed information about tRNA (adenine58-N1)-methyltransferase, go to the full flat file.
Word Map on EC 2.1.1.220
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2.1.1.220
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trnaimet
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1-methyladenosine
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nucleoside
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mtases
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two-subunit
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thermus
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exosome
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polyadenylation
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adomet
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t-loop
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polya
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s-adenosylmethionine
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n1-methylation
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heterotetramer
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homotetrameric
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methionyl-trna
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high-copy-number
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eubacteria
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methyltransferases
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s-adenosyl-l-homocysteine
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two-component
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s-adenosyl-l-methionine-dependent
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l-shaped
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tpsic
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trna-binding
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hypomodified
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drug development
- 2.1.1.220
- trnaimet
- 1-methyladenosine
- nucleoside
- mtases
-
two-subunit
-
thermus
-
exosome
-
polyadenylation
- adomet
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t-loop
- polya
- s-adenosylmethionine
-
n1-methylation
- heterotetramer
-
homotetrameric
- methionyl-trna
-
high-copy-number
- eubacteria
- methyltransferases
- s-adenosyl-l-homocysteine
-
two-component
-
s-adenosyl-l-methionine-dependent
-
l-shaped
-
tpsic
-
trna-binding
-
hypomodified
- drug development
Reaction
Synonyms
EC 2.1.1.36, Gcd10p, Gcd10p-Gcd14p, Gcd10pyGcd14p complex, Gcd14p, m1A58 MTase, m1A58 tRNA methyl-transferase, m1A58 tRNA methyltransferase, m1A58-methyltransferase, m1A58MTase, Rv2118c, Rv2118p, Trm6, TRM6-TRM61 holoenzyme, Trm61A, Trm61B, TRm61p, TRm6p, TrmI, tRNA (m1A58) methyltransferase, tRNA (m1A58) MTase, tRNA m(1)A58 methyltransferase, tRNA m1A58 methyltransferase, two component m1A58 tRNA methyl-transferase
ECTree
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Substrates Products
Substrates Products on EC 2.1.1.220 - tRNA (adenine58-N1)-methyltransferase
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REACTION DIAGRAM
2 S-adenosyl-L-methionine + adenine58 in tRNA
2 S-adenosyl-L-homocysteine + N1-methyladenine58 in tRNA
2 S-adenosyl-L-methionine + adenine58 in tRNALys3
2 S-adenosyl-L-homocysteine + N1-methyladenine58 in tRNALys3
S-adenosyl-L-methionine + adenine58 in human tRNA3Lys
S-adenosyl-L-homocysteine + N1-methyladenine58 in human tRNA3Lys
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?
S-adenosyl-L-methionine + adenine58 in initiator tRNAMet
S-adenosyl-L-homocysteine + N1-methyladenine58 in initiator tRNAMet
S-adenosyl-L-methionine + adenine58 in tRNA
S-adenosyl-L-homocysteine + N1-methyladenine58 in tRNA
S-adenosyl-L-methionine + adenine58 in tRNA3Lys
S-adenosyl-L-homocysteine + N1-methyladenine58 in tRNA3Lys
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-
-
?
S-adenosyl-L-methionine + adenine58 in tRNAGGUThr
S-adenosyl-L-homocysteine + N1-methyladenine58 in tRNA
S-adenosyl-L-methionine + adenine58 in tRNAPhe
S-adenosyl-L-homocysteine + N1-methyladenine58 in tRNAPhe
S-adenosyl-L-methionine + adenine58 in yeast initiator tRNAMet
S-adenosyl-L-homocysteine + N1-methyladenine58 in yeast initiator tRNAMet
no methylation of the A58U mutant of yeast initiator tRNA(Met)
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-
?
2 S-adenosyl-L-homocysteine + N1-methyladenine58 in tRNA
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-
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?
2 S-adenosyl-L-methionine + adenine58 in tRNA
2 S-adenosyl-L-homocysteine + N1-methyladenine58 in tRNA
P41814; P46959
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-
-
?
2 S-adenosyl-L-methionine + adenine58 in tRNA
2 S-adenosyl-L-homocysteine + N1-methyladenine58 in tRNA
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-
-
?
2 S-adenosyl-L-homocysteine + N1-methyladenine58 in tRNALys3
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-
-
?
2 S-adenosyl-L-methionine + adenine58 in tRNALys3
2 S-adenosyl-L-homocysteine + N1-methyladenine58 in tRNALys3
P41814; P46959
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-
-
?
S-adenosyl-L-methionine + adenine58 in initiator tRNAMet
S-adenosyl-L-homocysteine + N1-methyladenine58 in initiator tRNAMet
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-
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?
S-adenosyl-L-methionine + adenine58 in initiator tRNAMet
S-adenosyl-L-homocysteine + N1-methyladenine58 in initiator tRNAMet
P46959; P41814
the Gcd10pGcd14p complex is required specifically at the initiation step of translation because of a strong requirement for 1-methyladenosine at position 58 (m1A58) in the processing and accumulation of initiator tRNAMet
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-
?
S-adenosyl-L-methionine + adenine58 in initiator tRNAMet
S-adenosyl-L-homocysteine + N1-methyladenine58 in initiator tRNAMet
P46959; P41814
purified Flag-tagged Gcd14p alone had no enzymatic activity and is severely impaired for tRNA-binding compared with the wild-type complex, suggesting that Gcd10p is required for tight binding of the tRNA substrate
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?
S-adenosyl-L-homocysteine + N1-methyladenine58 in tRNA
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-
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?
S-adenosyl-L-methionine + adenine58 in tRNA
S-adenosyl-L-homocysteine + N1-methyladenine58 in tRNA
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-
-
?
S-adenosyl-L-methionine + adenine58 in tRNA
S-adenosyl-L-homocysteine + N1-methyladenine58 in tRNA
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tRNA m1A58 methyltransferase, TrmI, catalyzes the transfer of a methyl group from S-adenosyl-L-methionine to N1 of adenine 58 in the T-loop of tRNAs
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?
S-adenosyl-L-methionine + adenine58 in tRNA
S-adenosyl-L-homocysteine + N1-methyladenine58 in tRNA
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-
-
-
?
S-adenosyl-L-methionine + adenine58 in tRNA
S-adenosyl-L-homocysteine + N1-methyladenine58 in tRNA
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-
-
?
S-adenosyl-L-methionine + adenine58 in tRNA
S-adenosyl-L-homocysteine + N1-methyladenine58 in tRNA
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modification at the conserved nucleotide 58 in the TCC loop
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?
S-adenosyl-L-methionine + adenine58 in tRNA
S-adenosyl-L-homocysteine + N1-methyladenine58 in tRNA
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tRNA m1A58 methyltransferase, TrmI, catalyzes the transfer of a methyl group from S-adenosyl-L-methionine to N1 of adenine 58 in the T-loop of tRNAs
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-
?
S-adenosyl-L-methionine + adenine58 in tRNA
S-adenosyl-L-homocysteine + N1-methyladenine58 in tRNA
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-
-
-
?
S-adenosyl-L-methionine + adenine58 in tRNA
S-adenosyl-L-homocysteine + N1-methyladenine58 in tRNA
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specific for the A58 position. Rv2118p does not methylate other positions in tRNA to any significant extent
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-
?
S-adenosyl-L-methionine + adenine58 in tRNA
S-adenosyl-L-homocysteine + N1-methyladenine58 in tRNA
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tRNA m1A58 methyltransferase, TrmI, catalyzes the transfer of a methyl group from S-adenosyl-L-methionine to N1 of adenine 58 in the T-loop of tRNAs
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-
?
S-adenosyl-L-methionine + adenine58 in tRNA
S-adenosyl-L-homocysteine + N1-methyladenine58 in tRNA
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-
-
-
?
S-adenosyl-L-methionine + adenine58 in tRNA
S-adenosyl-L-homocysteine + N1-methyladenine58 in tRNA
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tRNA m1A58 methyltransferase, TrmI, catalyzes the transfer of a methyl group from S-adenosyl-L-methionine to N1 of adenine 58 in the T-loop of tRNAs
-
-
?
S-adenosyl-L-methionine + adenine58 in tRNA
S-adenosyl-L-homocysteine + N1-methyladenine58 in tRNA
-
-
-
-
?
S-adenosyl-L-methionine + adenine58 in tRNA
S-adenosyl-L-homocysteine + N1-methyladenine58 in tRNA
P41814; P46959
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-
-
?
S-adenosyl-L-methionine + adenine58 in tRNA
S-adenosyl-L-homocysteine + N1-methyladenine58 in tRNA
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TRM6 and TRM61 which compose a tRNA m1A58 methyltransferase that methylates m1A58 in tRNAs, especially tRNAiMe
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?
S-adenosyl-L-methionine + adenine58 in tRNA
S-adenosyl-L-homocysteine + N1-methyladenine58 in tRNA
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tRNA m1A58 methyltransferase, TrmI, catalyzes the transfer of a methyl group from S-adenosyl-L-methionine to N1 of adenine 58 in the T-loop of tRNAs
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-
?
S-adenosyl-L-methionine + adenine58 in tRNA
S-adenosyl-L-homocysteine + N1-methyladenine58 in tRNA
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adenine at position 58 is the most conserved nucleoside in tRNA and is located in the TpsiC loop
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?
S-adenosyl-L-methionine + adenine58 in tRNA
S-adenosyl-L-homocysteine + N1-methyladenine58 in tRNA
P41814; P46959
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-
-
?
S-adenosyl-L-methionine + adenine58 in tRNA
S-adenosyl-L-homocysteine + N1-methyladenine58 in tRNA
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-
-
-
?
S-adenosyl-L-methionine + adenine58 in tRNA
S-adenosyl-L-homocysteine + N1-methyladenine58 in tRNA
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tRNA m1A58 methyltransferase, TrmI, catalyzes the transfer of a methyl group from S-adenosyl-L-methionine to N1 of adenine 58 in the T-loop of tRNAs
-
-
?
S-adenosyl-L-methionine + adenine58 in tRNA
S-adenosyl-L-homocysteine + N1-methyladenine58 in tRNA
-
-
-
-
?
S-adenosyl-L-methionine + adenine58 in tRNA
S-adenosyl-L-homocysteine + N1-methyladenine58 in tRNA
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-
-
?
S-adenosyl-L-methionine + adenine58 in tRNA
S-adenosyl-L-homocysteine + N1-methyladenine58 in tRNA
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-
-
-
?
S-adenosyl-L-methionine + adenine58 in tRNA
S-adenosyl-L-homocysteine + N1-methyladenine58 in tRNA
catalyzes the sitespecific formation of m1A at position 58 of the T-loop of tRNA in the absence of any other complementary protein
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-
?
S-adenosyl-L-methionine + adenine58 in tRNA
S-adenosyl-L-homocysteine + N1-methyladenine58 in tRNA
-
tRNA m1A58 methyltransferase, TrmI, catalyzes the transfer of a methyl group from S-adenosyl-L-methionine to N1 of adenine 58 in the T-loop of tRNAs
-
-
?
S-adenosyl-L-methionine + adenine58 in tRNA
S-adenosyl-L-homocysteine + N1-methyladenine58 in tRNA
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-
-
?
S-adenosyl-L-methionine + adenine58 in tRNA
S-adenosyl-L-homocysteine + N1-methyladenine58 in tRNA
Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039
catalyzes the sitespecific formation of m1A at position 58 of the T-loop of tRNA in the absence of any other complementary protein
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?
S-adenosyl-L-homocysteine + N1-methyladenine58 in tRNA
the tRNA from Thermus thermophilus, that contains C60 instead of U60, is poorly methylated. Nucleoside analysis of tRNAGGUThr from the wild-type strain indicates that less than 50% of tRNAGGUThr contain m1A58
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?
S-adenosyl-L-methionine + adenine58 in tRNAGGUThr
S-adenosyl-L-homocysteine + N1-methyladenine58 in tRNA
the tRNA from Thermus thermophilus, that contains C60 instead of U60, is poorly methylated. Nucleoside analysis of tRNAGGUThr from the wild-type strain indicates that less than 50% of tRNAGGUThr contain m1A58
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?
S-adenosyl-L-homocysteine + N1-methyladenine58 in tRNAPhe
the Thermus thermophilus tRNAPhe transcript is methylated efficiently by the Thermus thermophilus enzyme, whereas the Saccharomyces cerevisiae tRNAPhe transcript is poorly methylated. Analysis of fourteen chimeric tRNA transcripts derived from these two tRNA reveals that enzyme TrmI recognized the combination of aminoacyl stem, variable region, and T-loop. TrmI methylates deltion transcripts still containing the aminoacyl stem, variable region, and T-arm. Positive sequence determinants are C56, purine 57, A58, and U60. Replacing A58 with inosine and 2-aminopurine completely abrogates methylation, demonstrating that the 6-amino group in A58 is recognized by enzyme TrmI
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?
S-adenosyl-L-methionine + adenine58 in tRNAPhe
S-adenosyl-L-homocysteine + N1-methyladenine58 in tRNAPhe
the Thermus thermophilus tRNAPhe transcript is methylated efficiently by the Thermus thermophilus enzyme, whereas the Saccharomyces cerevisiae tRNAPhe transcript is poorly methylated. Analysis of fourteen chimeric tRNA transcripts derived from these two tRNA reveals that enzyme TrmI recognized the combination of aminoacyl stem, variable region, and T-loop. TrmI methylates deltion transcripts still containing the aminoacyl stem, variable region, and T-arm. Positive sequence determinants are C56, purine 57, A58, and U60. Replacing A58 with inosine and 2-aminopurine completely abrogates methylation, demonstrating that the 6-amino group in A58 is recognized by enzyme TrmI
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?