2.1.1.218: tRNA (adenine9-N1)-methyltransferase

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For detailed information about tRNA (adenine9-N1)-methyltransferase, go to the full flat file.

Reaction

Synonyms

adenosine-specific Trm10, EC 2.1.1.36, hTRMT10B, m1A9 MTase, m1R9-specific TkTrm10, More, SaTrm10, SPOUT MTase, TK0422p, TkTrm10, Trm10, Trm10p (ambiguous), TRMT10B, Trmt10C, tRNA m1A9 methyltransferase, tRNA m1A9 MTase, tRNA m1A9-methyltransferase, tRNA m1R9 methyltransferase, tRNA(m1G9/m1A9)-methyltransferase, tRNA(m1G9/m1A9)MTase

ECTree

2 Transferases

2.1 Transferring one-carbon groups

2.1.1 Methyltransferases

2.1.1.218 tRNA (adenine9-N1)-methyltransferase

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Results	in table
664	AA Sequence
4	Cloned(Commentary)
6	Cofactor
3	Crystallization (Commentary)
4	Disease/ Diagnostics
42	General Information
2	KM Value [mM]
1	Localization
3	Metals/Ions
2	Molecular Weight [Da]
20	Natural Substrates/ Products (Substrates)
23	Organism
13	PDB ID
4	pH Optimum
2	pH Range
35	Protein Variants
4	Purification (Commentary)
1	Reaction
19	Reference
41	Substrates and Products (Substrate)
6	Subunits
48	Synonyms
1	Systematic Name
4	Temperature Optimum [°C]
2	Turnover Number [1/s]

Cloned

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Cloned on EC 2.1.1.218 - tRNA (adenine9-N1)-methyltransferase

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expression in Escherichia coli

2 entries

gene TK0422, sequence comparisons, recombinant expression of N-terminally His6-tagged protein in Escherichia coli

Thermococcus kodakarensis

Q5JD38

756865

recombinant expression of His-tagged full-length wild-type enzyme, of truncated enzyme SaTrm10 1-249, and of enzyme mutants in Escherichia coli strain Rosetta (DE3)pLysS, recombinant expression of nontagged wild-type enzyme

Sulfolobus acidocaldarius

Q4J894

757838