2.1.1.217: tRNA (adenine22-N1)-methyltransferase

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For detailed information about tRNA (adenine22-N1)-methyltransferase, go to the full flat file.

Word Map on EC 2.1.1.217

2.1.1.217

mtases

methyltransferases

adomet

rna-binding

groove

sam-dependent

class-i

ligand-free

Reaction

Synonyms

BsTrmK, Class I MTase, EC 2.1.1.36, m1A22 MTase, m1A22 tRNA methyltransferase, Sp1610, TrmK, tRNA: m1A22 methyltransferase, yqfN

ECTree

2 Transferases

2.1 Transferring one-carbon groups

2.1.1 Methyltransferases

2.1.1.217 tRNA (adenine22-N1)-methyltransferase

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Results	in table
1	Activating Compound
879	AA Sequence
4	Cloned(Commentary)
6	Cofactor
2	Crystallization (Commentary)
21	General Information
3	KM Value [mM]
1	Metals/Ions
13	Natural Substrates/ Products (Substrates)
14	Organism
9	PDB ID
1	pH Optimum
2	Protein Variants
4	Purification (Commentary)
1	Reaction
12	Reference
21	Substrates and Products (Substrate)
7	Subunits
26	Synonyms
1	Systematic Name
2	Temperature Optimum [°C]

Purification

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Purification on EC 2.1.1.217 - tRNA (adenine22-N1)-methyltransferase

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2 entries

recombinant His-tagged enzyme by nickel affinity chromatography

Bacillus subtilis

P54471

757861

recombinant N-terminally His6-tagged mutant enzyme C35S/C152S from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, tag cleavage by thrombin, followed by ultrafiltration and gel filtration

Bacillus subtilis

P54471

756149