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2.1.1.217: tRNA (adenine22-N1)-methyltransferase

This is an abbreviated version!
For detailed information about tRNA (adenine22-N1)-methyltransferase, go to the full flat file.

Word Map on EC 2.1.1.217

Reaction

S-adenosyl-L-methionine
+
adenine22 in tRNA
=
S-adenosyl-L-homocysteine
 +
N1-methyladenine22 in tRNA

Synonyms

BsTrmK, Class I MTase, EC 2.1.1.36, m1A22 MTase, m1A22 tRNA methyltransferase, Sp1610, TrmK, tRNA: m1A22 methyltransferase, yqfN

ECTree

     2 Transferases
         2.1 Transferring one-carbon groups
             2.1.1 Methyltransferases
                2.1.1.217 tRNA (adenine22-N1)-methyltransferase

Crystallization

Crystallization on EC 2.1.1.217 - tRNA (adenine22-N1)-methyltransferase

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CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
sitting drop vapor diffusion method, mixing of 0.0016 ml of 3 mg/ml protein in 50 mM Tris-HCl, pH 8.0, 500 mM NaCl, and 2% glycerol, with crystallization solution containing 0.2 M ammonium acetate, 0.1 M sodium acetate trihydrate, pH 5.0, 30% w/v PEG 4000, and 8% 2-methyl-2,4-pentanediol, and equuilibration against 0.1 ml reservoir solution, microseeding, 4°C, X-ray diffraction structure determination and analysis, molecular replacement using structures of sp1610 (Streptococcus pneumoniae orthologue of BsTrmK, PDB code 3KR9), LMOf2365 1472 (Listeria Monocytogens, PDB code 3GNL) and of SAG1203 (Streptococcus agalactiae, PDB code: 3LEC) as templates, modelling
crystal structure of Sp1610 in the ligand-free and the AdoMet-bound forms at resolutions of 2.0 and 3.0 A, microbatch method