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2.1.1.216: tRNA (guanine26-N2)-dimethyltransferase

This is an abbreviated version!
For detailed information about tRNA (guanine26-N2)-dimethyltransferase, go to the full flat file.

Word Map on EC 2.1.1.216

Reaction

2 S-adenosyl-L-methionine +

guanine26 in tRNA
= 2 S-adenosyl-L-homocysteine +
N2-dimethylguanine26 in tRNA

Synonyms

EC 2.1.1.32, Ta0997, transfer RNA methyltransferase, Trm1, Trm1 methyltransferase, Trm1p, tRNA (guanine(26)-N(2))-dimethyltransferase, tRNA (m22G26)dimethyltransferase, tRNA(guanine-26,N2-N2) methyltransferase, tRNA(guanine26,N2,N2)-dimethyltransferase, tRNA(m22G26)dimethyltransferase, ZC376.5

ECTree

     2 Transferases
         2.1 Transferring one-carbon groups
             2.1.1 Methyltransferases
                2.1.1.216 tRNA (guanine26-N2)-dimethyltransferase

Purification

Purification on EC 2.1.1.216 - tRNA (guanine26-N2)-dimethyltransferase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
native and mutant Trm1 proteins
recombinant enzyme (pfTrm1p) with a His6-tag at the N-terminus
-
recombinant enzyme from Escherichia coli strain BL21 (DE3) Rosetta 2