2.1.1.205: tRNA (cytidine32/guanosine34-2'-O)-methyltransferase
This is an abbreviated version!
For detailed information about tRNA (cytidine32/guanosine34-2'-O)-methyltransferase, go to the full flat file.
Reaction
Synonyms
trm7, Trm7p
ECTree
2.1.1.205 tRNA (cytidine32/guanosine34-2'-O)-methyltransferaseAdvanced search results
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results (Substrates Products
Substrates Products on EC 2.1.1.205 - tRNA (cytidine32/guanosine34-2'-O)-methyltransferase
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SUBSTRATE 
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + cytidine32/guanosine34 in tRNA
S-adenosyl-L-homocysteine + 2'-O-methylcytidine32/2'-O-methylguanosine34 in tRNA
S-adenosyl-L-methionine + cytidine32/guanosine34 in tRNALeu
S-adenosyl-L-homocysteine + 2'-O-methylcytidine32/2'-O methylguanosine34 in tRNALeu
S-adenosyl-L-methionine + cytidine32/guanosine34 in tRNAPhe
S-adenosyl-L-homocysteine + 2'-O-methylcytidine32/2'-O methylguanosine34 in tRNAPhe
S-adenosyl-L-methionine + cytidine32/guanosine34 in tRNATrp
S-adenosyl-L-homocysteine + 2'-O-methylcytidine32/2'-O methylguanosine34 in tRNATrp
S-adenosyl-L-methionine + cytidine32/guanosine34 in tRNA
S-adenosyl-L-homocysteine + 2'-O-methylcytidine32/2'-O-methylguanosine34 in tRNA
the enzyme catalyses the formation of 2'-O-methylnucleotides at positions 32 and 34 of the yeast tRNAPhe, tRNATrp and, possibly, tRNALeu
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?
S-adenosyl-L-methionine + cytidine32/guanosine34 in tRNA
S-adenosyl-L-homocysteine + 2'-O-methylcytidine32/2'-O-methylguanosine34 in tRNA
Trm7p methylates the 2'-O-ribose of nucleotides at positions 32 and 34 of the tRNA anticodon loop, both in vivo and in vitro
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?
S-adenosyl-L-methionine + cytidine32/guanosine34 in tRNALeu
S-adenosyl-L-homocysteine + 2'-O-methylcytidine32/2'-O methylguanosine34 in tRNALeu
enzyme complex Trm7-Trm734
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-
?
S-adenosyl-L-methionine + cytidine32/guanosine34 in tRNALeu
S-adenosyl-L-homocysteine + 2'-O-methylcytidine32/2'-O methylguanosine34 in tRNALeu
enzyme complex Trm7-Trm734
-
-
?
S-adenosyl-L-methionine + cytidine32/guanosine34 in tRNAPhe
S-adenosyl-L-homocysteine + 2'-O-methylcytidine32/2'-O methylguanosine34 in tRNAPhe
enzyme complex Trm7-Trm734
-
-
?
S-adenosyl-L-methionine + cytidine32/guanosine34 in tRNAPhe
S-adenosyl-L-homocysteine + 2'-O-methylcytidine32/2'-O methylguanosine34 in tRNAPhe
enzyme complex Trm7-Trm734
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-
?
S-adenosyl-L-methionine + cytidine32/guanosine34 in tRNATrp
S-adenosyl-L-homocysteine + 2'-O-methylcytidine32/2'-O methylguanosine34 in tRNATrp
enzyme complex Trm7-Trm734
-
-
?
S-adenosyl-L-methionine + cytidine32/guanosine34 in tRNATrp
S-adenosyl-L-homocysteine + 2'-O-methylcytidine32/2'-O methylguanosine34 in tRNATrp
enzyme complex Trm7-Trm734
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?
additional information
?
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the enzymatic activity of Trm7-Trm734 is measured by incorporation of 14C-methyl groups from [methyl-14C]-SAM into tRNA transcripts. Although these mutant tRNAPhe transcripts are methylated by Trm7-Trm734, the substitution of G34 by C or U (G34C or G34U) strongly enhances the methyl-group acceptance activity. Trm7-Trm734 preferentially methylates pyrimidine nucleoside at position 34 (pyrimidine34) rather than purine nucleoside. Of the 43 tRNA molecular species in Saccharomyces cerevisiae, only tRNAPhe, tRNATrp and tRNALeu possess the combination of Cm32, m1G37 and/or pyrimidine34 in the normal anticodon-loop, which is required for the effective methylation by Trm7-Trm734
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additional information
?
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the enzymatic activity of Trm7-Trm734 is measured by incorporation of 14C-methyl groups from [methyl-14C]-SAM into tRNA transcripts. Although these mutant tRNAPhe transcripts are methylated by Trm7-Trm734, the substitution of G34 by C or U (G34C or G34U) strongly enhances the methyl-group acceptance activity. Trm7-Trm734 preferentially methylates pyrimidine nucleoside at position 34 (pyrimidine34) rather than purine nucleoside. Of the 43 tRNA molecular species in Saccharomyces cerevisiae, only tRNAPhe, tRNATrp and tRNALeu possess the combination of Cm32, m1G37 and/or pyrimidine34 in the normal anticodon-loop, which is required for the effective methylation by Trm7-Trm734
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