2.1.1.204: tRNA (cytosine38-C5)-methyltransferase
This is an abbreviated version!
For detailed information about tRNA (cytosine38-C5)-methyltransferase, go to the full flat file.
Word Map on EC 2.1.1.204
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2.1.1.204
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methyltransferases
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n6-methyladenosine
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5-methylcytosine
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mtases
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2'-o-methylation
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epitranscriptomic
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methyltransferase-like
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2'-o-methyltransferase
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piwi-interacting
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mettl16
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spout
- 2.1.1.204
- methyltransferases
- n6-methyladenosine
- 5-methylcytosine
- mtases
-
2'-o-methylation
-
epitranscriptomic
-
methyltransferase-like
-
2'-o-methyltransferase
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piwi-interacting
- mettl16
-
spout
Reaction
Synonyms
(cytosine-5) RNA methyltransferase, cytosine-5 tRNA methyltransferase, dDnmt2, DNA methyltransferase 2, DnmA, Dnmt2, DNMT2 methyltransferase, EC 2.1.1.29, hDNMT2, Pf-DNMT2, PMT1, pombe methyltransferase 1, RCMT, RNA methyltransferase, spDnmt2, transfer RNA aspartic acid methyltransferase 1, TRDMT1, tRNA aspartic acid methyltransferase 1, tRNA-aspartic acid methyltransferase 1
ECTree
2.1.1.204 tRNA (cytosine38-C5)-methyltransferaseAdvanced search results
results (
results (Substrates Products
Substrates Products on EC 2.1.1.204 - tRNA (cytosine38-C5)-methyltransferase
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SUBSTRATE 
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + cytosine38 in G34tRNAAsp
S-adenosyl-L-homocysteine + 5-methylcytosine38 in G34tRNAAsp
S-adenosyl-L-methionine + cytosine38 in Q34tRNAAsp
S-adenosyl-L-homocysteine + 5-methylcytosine38 in Q34tRNAAsp
S-adenosyl-L-methionine + cytosine38 in tRNA
S-adenosyl-L-homocysteine + 5-methylcytosine38 in tRNA
S-adenosyl-L-methionine + cytosine38 in tRNA-Asp(GUC)
S-adenosyl-L-homocysteine + 5-methylcytosine38 in tRNA-Asp(GUC)
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-
-
?
S-adenosyl-L-methionine + cytosine38 in tRNAAsp
S-adenosyl-L-homocysteine + 5-methylcytosine38 in tRNAAsp
S-adenosyl-L-methionine + cytosine38 in tRNAAsp precursor
S-adenosyl-L-homocysteine + 5-methylcytosine38 in tRNAAsp precursor
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-
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?
S-adenosyl-L-methionine + cytosine38 in tRNAAsp(GTC)
S-adenosyl-L-homocysteine + 5-methylcytosine38 in tRNAAsp(GTC)
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-
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?
S-adenosyl-L-methionine + cytosine38 in tRNAAspGTC
S-adenosyl-L-homocysteine + 5-methylcytosine38 in tRNAAspGTC
S-adenosyl-L-methionine + cytosine38 in tRNAGlu
S-adenosyl-L-homocysteine + 5-methylcytosine38 in tRNAGlu
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25% of the activity with tRNAAsp, the activity with tRNAGlu is not affected by the presence of peptone
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-
?
S-adenosyl-L-methionine + cytosine38 in tRNAGlu
S-adenosyl-L-homocysteine + 5-methylcytosine38 in tRNGlu
S-adenosyl-L-methionine + cytosine38 in tRNAGly precursor
S-adenosyl-L-homocysteine + 5-methylcytosine38 in tRNAGly precursor
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-
-
-
?
S-adenosyl-L-methionine + cytosine38 in tRNAGly(GCC)
S-adenosyl-L-homocysteine + 5-methylcytosine38 in tRNAGly(GCC)
S-adenosyl-L-methionine + cytosine38 in tRNAGlyGCC
S-adenosyl-L-homocysteine + 5-methylcytosine38 in tRNAGlyGCC
S-adenosyl-L-methionine + cytosine38 in tRNAVal
S-adenosyl-L-homocysteine + 5-methylcytosine38 in tRNAVal
assay with tritium-labeled SAM, very low activity
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-
?
S-adenosyl-L-methionine + cytosine38 in tRNAVal precursor
S-adenosyl-L-homocysteine + 5-methylcytosine38 in tRNAVal precursor
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-
-
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?
S-adenosyl-L-methionine + cytosine38 in tRNAVal(AAC)
S-adenosyl-L-homocysteine + 5-methylcytosine38 in tRNAVal(AAC)
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-
-
?
S-adenosyl-L-methionine + cytosine38 in tRNAValAAC
S-adenosyl-L-homocysteine + 5-methylcytosine38 in tRNAValAAC
S-adenosyl-L-methionine + cytosine38 in G34tRNAAsp
S-adenosyl-L-homocysteine + 5-methylcytosine38 in G34tRNAAsp
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-
-
?
S-adenosyl-L-methionine + cytosine38 in G34tRNAAsp
S-adenosyl-L-homocysteine + 5-methylcytosine38 in G34tRNAAsp
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-
-
?
S-adenosyl-L-methionine + cytosine38 in G34tRNAAsp
S-adenosyl-L-homocysteine + 5-methylcytosine38 in G34tRNAAsp
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-
-
?
S-adenosyl-L-methionine + cytosine38 in Q34tRNAAsp
S-adenosyl-L-homocysteine + 5-methylcytosine38 in Q34tRNAAsp
preferred substrate
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-
?
S-adenosyl-L-methionine + cytosine38 in Q34tRNAAsp
S-adenosyl-L-homocysteine + 5-methylcytosine38 in Q34tRNAAsp
preferred substrate
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-
?
S-adenosyl-L-methionine + cytosine38 in Q34tRNAAsp
S-adenosyl-L-homocysteine + 5-methylcytosine38 in Q34tRNAAsp
preferred substrate
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-
?
S-adenosyl-L-methionine + cytosine38 in tRNA
S-adenosyl-L-homocysteine + 5-methylcytosine38 in tRNA
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-
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?
S-adenosyl-L-methionine + cytosine38 in tRNA
S-adenosyl-L-homocysteine + 5-methylcytosine38 in tRNA
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-
-
?
S-adenosyl-L-methionine + cytosine38 in tRNA
S-adenosyl-L-homocysteine + 5-methylcytosine38 in tRNA
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-
-
?
S-adenosyl-L-methionine + cytosine38 in tRNA
S-adenosyl-L-homocysteine + 5-methylcytosine38 in tRNA
RNA methylation by Dnmt2 protects tRNAs against stress-induced cleavage
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-
?
S-adenosyl-L-methionine + cytosine38 in tRNA
S-adenosyl-L-homocysteine + 5-methylcytosine38 in tRNA
methylation of cytosine 38 in the anti-codon lopp of tRNAAsp(GTC), tRNAVal(AAC) and tRNAGly(GCC). Other C38-containing tRNAs-including tRNAMet(ATG), tRNAGlu(CTC), and tRNAHis(GTG) are not detectably methylated in a Dnmt2-dependent manner
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?
S-adenosyl-L-methionine + cytosine38 in tRNA
S-adenosyl-L-homocysteine + 5-methylcytosine38 in tRNA
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-
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?
S-adenosyl-L-methionine + cytosine38 in tRNA
S-adenosyl-L-homocysteine + 5-methylcytosine38 in tRNA
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in vitro transcribed tRNAAsp is methylated by DNMT2, albeit at reduced efficiency. Human, mouse, and Dictyostelium tRNA all are substrates for human DNMT2. C79, E119, R160 and R162 are essential for the catalytic mechanism of DNMT2
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?
S-adenosyl-L-methionine + cytosine38 in tRNA
S-adenosyl-L-homocysteine + 5-methylcytosine38 in tRNA
methylates tRNAAsp specifically at cytosine38 in the anticodon loop. Human DNMT2 protein restores methylation in vitro to tRNAAsp from Dnmt2-deficient strains of mouse, Arabidopsis thaliana, and Drosophila melanogaster in a manner that is dependent on preexisting patterns of modified nucleosides. Unmodified tRNAAsp produced by in vitro transcription is not a substrate for DNMT2, which suggests that methylation is guided to cytosine38 by other modifications. Mannosylqueuosine is likely to be involved, because it is unique to tRNAAsp. Analysis of tRNAAsp sequences show complete conservation of the anticodon loop in species whose genomes encode Dnmt2 homologs, but the tRNAAsp anticodon loops in Caenorhabditis elegans and Saccharomyces cerevisiae, which lack Dnmt2 homologs, have diverged
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?
S-adenosyl-L-methionine + cytosine38 in tRNA
S-adenosyl-L-homocysteine + 5-methylcytosine38 in tRNA
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?
S-adenosyl-L-methionine + cytosine38 in tRNA
S-adenosyl-L-homocysteine + 5-methylcytosine38 in tRNA
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-
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?
S-adenosyl-L-methionine + cytosine38 in tRNA
S-adenosyl-L-homocysteine + 5-methylcytosine38 in tRNA
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-
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?
S-adenosyl-L-methionine + cytosine38 in tRNA
S-adenosyl-L-homocysteine + 5-methylcytosine38 in tRNA
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-
-
?
S-adenosyl-L-methionine + cytosine38 in tRNAAsp
S-adenosyl-L-homocysteine + 5-methylcytosine38 in tRNAAsp
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?
S-adenosyl-L-methionine + cytosine38 in tRNAAsp
S-adenosyl-L-homocysteine + 5-methylcytosine38 in tRNAAsp
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-
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?
S-adenosyl-L-methionine + cytosine38 in tRNAAsp
S-adenosyl-L-homocysteine + 5-methylcytosine38 in tRNAAsp
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-
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?
S-adenosyl-L-methionine + cytosine38 in tRNAAsp
S-adenosyl-L-homocysteine + 5-methylcytosine38 in tRNAAsp
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-
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?
S-adenosyl-L-methionine + cytosine38 in tRNAAsp
S-adenosyl-L-homocysteine + 5-methylcytosine38 in tRNAAsp
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-
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?
S-adenosyl-L-methionine + cytosine38 in tRNAAsp
S-adenosyl-L-homocysteine + 5-methylcytosine38 in tRNAAsp
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mapping of the tRNA binding site of DNMT2 by systematically mutating surface-exposed lysine and arginine residues to alanine and studying the tRNA methylation activity and binding of the corresponding variants. tRNA specificity determinants and tRNA binding pocket structure in the DNMT2, overview
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?
S-adenosyl-L-methionine + cytosine38 in tRNAAsp
S-adenosyl-L-homocysteine + 5-methylcytosine38 in tRNAAsp
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-
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?
S-adenosyl-L-methionine + cytosine38 in tRNAAsp
S-adenosyl-L-homocysteine + 5-methylcytosine38 in tRNAAsp
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-
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-
?
S-adenosyl-L-methionine + cytosine38 in tRNAAsp
S-adenosyl-L-homocysteine + 5-methylcytosine38 in tRNAAsp
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-
-
?
S-adenosyl-L-methionine + cytosine38 in tRNAAsp
S-adenosyl-L-homocysteine + 5-methylcytosine38 in tRNAAsp
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-
-
?
S-adenosyl-L-methionine + cytosine38 in tRNAAsp
S-adenosyl-L-homocysteine + 5-methylcytosine38 in tRNAAsp
assay with tritium-labeled SAM, specific for tRNAAsp
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-
?
S-adenosyl-L-methionine + cytosine38 in tRNAAsp
S-adenosyl-L-homocysteine + 5-methylcytosine38 in tRNAAsp
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-
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?
S-adenosyl-L-methionine + cytosine38 in tRNAAsp
S-adenosyl-L-homocysteine + 5-methylcytosine38 in tRNAAsp
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-
-
?
S-adenosyl-L-methionine + cytosine38 in tRNAAsp
S-adenosyl-L-homocysteine + 5-methylcytosine38 in tRNAAsp
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?
S-adenosyl-L-methionine + cytosine38 in tRNAAsp
S-adenosyl-L-homocysteine + 5-methylcytosine38 in tRNAAsp
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-
-
?
S-adenosyl-L-methionine + cytosine38 in tRNAAsp
S-adenosyl-L-homocysteine + 5-methylcytosine38 in tRNAAsp
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-
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?
S-adenosyl-L-methionine + cytosine38 in tRNAAspGTC
S-adenosyl-L-homocysteine + 5-methylcytosine38 in tRNAAspGTC
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?
S-adenosyl-L-methionine + cytosine38 in tRNAAspGTC
S-adenosyl-L-homocysteine + 5-methylcytosine38 in tRNAAspGTC
Dnmt2-mediated methylation at position C38 in the anticodon loop of tRNA-Asp may have a role in the discrimination between Asp and Glu near-cognate codons
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?
S-adenosyl-L-methionine + cytosine38 in tRNAAspGTC
S-adenosyl-L-homocysteine + 5-methylcytosine38 in tRNAAspGTC
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-
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?
S-adenosyl-L-methionine + cytosine38 in tRNAAspGTC
S-adenosyl-L-homocysteine + 5-methylcytosine38 in tRNAAspGTC
Dnmt2-mediated methylation at position C38 in the anticodon loop of tRNA-Asp may have a role in the discrimination between Asp and Glu near-cognate codons
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-
?
S-adenosyl-L-methionine + cytosine38 in tRNAGlu
S-adenosyl-L-homocysteine + 5-methylcytosine38 in tRNGlu
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-
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?
S-adenosyl-L-methionine + cytosine38 in tRNAGlu
S-adenosyl-L-homocysteine + 5-methylcytosine38 in tRNGlu
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-
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?
S-adenosyl-L-methionine + cytosine38 in tRNAGly(GCC)
S-adenosyl-L-homocysteine + 5-methylcytosine38 in tRNAGly(GCC)
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?
S-adenosyl-L-methionine + cytosine38 in tRNAGly(GCC)
S-adenosyl-L-homocysteine + 5-methylcytosine38 in tRNAGly(GCC)
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?
S-adenosyl-L-methionine + cytosine38 in tRNAGlyGCC
S-adenosyl-L-homocysteine + 5-methylcytosine38 in tRNAGlyGCC
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?
S-adenosyl-L-methionine + cytosine38 in tRNAGlyGCC
S-adenosyl-L-homocysteine + 5-methylcytosine38 in tRNAGlyGCC
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-
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?
S-adenosyl-L-methionine + cytosine38 in tRNAValAAC
S-adenosyl-L-homocysteine + 5-methylcytosine38 in tRNAValAAC
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?
S-adenosyl-L-methionine + cytosine38 in tRNAValAAC
S-adenosyl-L-homocysteine + 5-methylcytosine38 in tRNAValAAC
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?
additional information
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the enzyme DNMT2 is responsible for methylation of cytosine 38 in the anticodon loop of aspartic acid transfer RNA instead of transferring methyl group to the cytosine residues of DNA
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?
additional information
?
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Dnmt2 enzymes are cytosine-5 methyltransferases that methylate C38 of several tRNAs
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?
additional information
?
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Dnmt2 enzymes are transfer RNA (tRNA) methyltransferases for cytosine-5 methylation, foremost C38 (m5C38) of tRNAAsp. Dnmt2 binds to viral RNA
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additional information
?
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DNMT2 induces a conformational change in the tRNA of the DNMT2-tRNA complex
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?
additional information
?
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DNMT2 functions primarily, if not exclusively, as a cytosine 5-methylation RNA methyltransferase with three verified tRNA targets: tRNAAsp, tRNAGly and tRNAVal
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?
additional information
?
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the enzyme DNMT2 is responsible for methylation of cytosine 38 in the anticodon loop of aspartic acid transfer RNA instead of transferring methyl group to the cytosine residues of DNA
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?
additional information
?
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development and evaluation of 5-azacytidine-mediated RNA immunoprecipitation, a mechanism-based technique in nine steps that exploits the covalent bond formed between an RNA methyltransferase and the cytidine analogue 5-azacytidine to recover RNA targets by immunoprecipitation, overview. High frequency of C>G transversions at the cytosine residues targeted by the enzyme, allowing identification of the specific methylated cytosine(s) in target RNAs. tRNAGly(GCC) bears one DNMT2 target site (C38)
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?
additional information
?
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DNMT2 is able to methylate the cytosine 38 in the anticodon loop of aspartic acid transfer RNA instead of transferring methyl groups to the cytosine residues in the genomic DNA
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additional information
?
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Dnmt2 methylates C38 of tRNA in the bone marrow and modulates the stability and fragmentation of substrate tRNAs
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?
additional information
?
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the enzyme DNMT2 is responsible for methylation of cytosine 38 in the anticodon loop of aspartic acid transfer RNA instead of transferring methyl group to the cytosine residues of DNA
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?
additional information
?
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Dnmt2 enzymes are transfer RNA (tRNA) methyltransferases for cytosine-5 methylation, foremost C38 (m5C38) of tRNAAsp. Dnmt2 binds to viral RNA
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additional information
?
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DNMT2 is able to methylate the cytosine 38 in the anticodon loop of aspartic acid transfer RNA instead of transferring methyl groups to the cytosine residues in the genomic DNA
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additional information
?
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Dnmt2 methylates C38 of tRNA in the bone marrow and modulates the stability and fragmentation of substrate tRNAs
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?
additional information
?
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the DNA methyltransferase homologue TRDMT1 in Plasmodium falciparum specifically methylates endogenous aspartic acid tRNA
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additional information
?
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no activity with DNA and other tRNA substrates. Plasmodium falciparum has two isodecoders for valine tRNA, and the very low activity is seen on valine 1 substrate, whereas no activity can be detected on valine 2 tRNA substrate. Purified Pf-TRDMT1 methylates tRNA-Asp at C38 position
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additional information
?
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Dnmt2 enzymes are cytosine-5 methyltransferases that methylate C38 of several tRNAs. Pmt1 shows a striking selectivity for tRNAAsp methylation, which distinguishes Pmt1 from other Dnmt2 homologues. C34 of tRNAPro is a Pmt1- and queuosine-independent tRNA methylation site in Schizosaccharomyces pombe
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?
additional information
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in vitro methylation activity on tRNAAsp and, to a lesser extent, tRNAGlu. No methylation activity with C38-containing tRNAs for histidine, glutamate, and valine
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?
additional information
?
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Dnmt2 enzymes are transfer RNA (tRNA) methyltransferases for cytosine-5 methylation, foremost C38 (m5C38) of tRNAAsp
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additional information
?
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Q34tRNAAsp exhibits an only slightly increased affinity for Dnmt2 in comparison to unmodified G34tRNA
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additional information
?
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Dnmt2 enzymes are transfer RNA (tRNA) methyltransferases for cytosine-5 methylation, foremost C38 (m5C38) of tRNAAsp
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additional information
?
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Q34tRNAAsp exhibits an only slightly increased affinity for Dnmt2 in comparison to unmodified G34tRNA
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additional information
?
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Dnmt2 enzymes are transfer RNA (tRNA) methyltransferases for cytosine-5 methylation, foremost C38 (m5C38) of tRNAAsp
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additional information
?
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Q34tRNAAsp exhibits an only slightly increased affinity for Dnmt2 in comparison to unmodified G34tRNA
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