2.1.1.204: tRNA (cytosine38-C5)-methyltransferase
This is an abbreviated version!
For detailed information about tRNA (cytosine38-C5)-methyltransferase, go to the full flat file.
Word Map on EC 2.1.1.204
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2.1.1.204
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methyltransferases
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n6-methyladenosine
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5-methylcytosine
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mtases
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2'-o-methylation
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epitranscriptomic
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methyltransferase-like
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2'-o-methyltransferase
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piwi-interacting
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mettl16
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spout
- 2.1.1.204
- methyltransferases
- n6-methyladenosine
- 5-methylcytosine
- mtases
-
2'-o-methylation
-
epitranscriptomic
-
methyltransferase-like
-
2'-o-methyltransferase
-
piwi-interacting
- mettl16
-
spout
Reaction
Synonyms
(cytosine-5) RNA methyltransferase, cytosine-5 tRNA methyltransferase, dDnmt2, DNA methyltransferase 2, DnmA, Dnmt2, DNMT2 methyltransferase, EC 2.1.1.29, hDNMT2, Pf-DNMT2, PMT1, pombe methyltransferase 1, RCMT, RNA methyltransferase, spDnmt2, transfer RNA aspartic acid methyltransferase 1, TRDMT1, tRNA aspartic acid methyltransferase 1, tRNA-aspartic acid methyltransferase 1
ECTree
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Purification
Purification on EC 2.1.1.204 - tRNA (cytosine38-C5)-methyltransferase
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recombinant GST-tagged enzyme from Escherichia coli strain BL21(DE3) by ultracentrifugation, glutathione affinity chromatography, and tag cleavage through PreScission protease, followed by heparin affinity chromatography, gel filtration, and ultrafiltration
recombinant His-tagged enzyme from Escherichia coli strain Rosetta2 (DE3) by nickel affinity chromatography and dialysis
recombinant His6-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3)
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recombinant His6-tagged wild-type and mutant enzymes from Escherichia coli to over 90% purity, except for mutant R84A, which is about 75% pure
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