2.1.1.200: tRNA (cytidine32/uridine32-2'-O)-methyltransferase
This is an abbreviated version!
For detailed information about tRNA (cytidine32/uridine32-2'-O)-methyltransferase, go to the full flat file.
Reaction
Synonyms
2'-O-tRNA methyltransferase, PA14 14690, Saci_0621, TrMet(Xm32), TrmJ, tRNA (cytidine(32)/uridine(32)/adenosine(32)-2'-O)-methyltransferase, tRNA:Cm32/Um32 methyltransferase, tRNA:Cm32/Um32/Am32 methyltransferase, YfhQ
ECTree
2.1.1.200 tRNA (cytidine32/uridine32-2'-O)-methyltransferaseAdvanced search results
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results (Substrates Products
Substrates Products on EC 2.1.1.200 - tRNA (cytidine32/uridine32-2'-O)-methyltransferase
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SUBSTRATE 
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + adenine32 in tRNAPro(GGG)
S-adenosyl-L-homocysteine + 2'-O-methyladenine32 in tRNAPro(GGG)
S-adenosyl-L-methionine + cytidine32 in tRNA
S-adenosyl-L-homocysteine + 2'-O-methylcytidine32 in tRNA
S-adenosyl-L-methionine + cytidine32 in tRNAMet(CAU)
S-adenosyl-L-homocysteine + 2'-O-methylcytisine32 in tRNA
S-adenosyl-L-methionine + cytidine32 in tRNATrp(CCA)
S-adenosyl-L-homocysteine + 2'-O-methylcytidine32 in tRNATrp(CCA)
S-adenosyl-L-methionine + nucleoside32 in EctRNAfMet1(CAU)
S-adenosyl-L-homocysteine + 2'-O-methylnucleoside32 in EctRNAfMet1(CAU)
S-adenosyl-L-methionine + nucleoside32 in EctRNAfMet2(CAU)
S-adenosyl-L-homocysteine + 2'-O-methylnucleoside32 in EctRNAfMet2(CAU)
S-adenosyl-L-methionine + nucleoside32 in EctRNAGln1(UUG)
S-adenosyl-L-homocysteine + 2'-O-methylnucleoside32 in EctRNAGln1(UUG)
S-adenosyl-L-methionine + nucleoside32 in EctRNAGln2(CUG)
S-adenosyl-L-homocysteine + 2'-O-methylnucleoside32 in EctRNAGln2(CUG)
-
-
-
?
S-adenosyl-L-methionine + nucleoside32 in EctRNASer1(UGA)
S-adenosyl-L-homocysteine + 2'-O-methylnucleoside32 in EctRNASer1(UGA)
-
-
-
?
S-adenosyl-L-methionine + nucleoside32 in EctRNATrp1(CCA)
S-adenosyl-L-homocysteine + 2'-O-methylnucleoside32 in EctRNATrp1(CCA)
-
-
-
?
S-adenosyl-L-methionine + nucleoside32 in tRNA
S-adenosyl-L-homocysteine + 2'-O-methylnucleoside32 in tRNA
S-adenosyl-L-methionine + uridine32 in tRNA
S-adenosyl-L-homocysteine + 2'-O-methyluridine32 in tRNA
S-adenosyl-L-methionine + uridine32 in tRNAGln(UUG)
S-adenosyl-L-homocysteine + 2'-O-methyluridine32 in tRNAGln(UUG)
-
-
-
?
S-adenosyl-L-methionine + uridine32 in tRNAPro(CGG)
S-adenosyl-L-homocysteine + 2'-O-methyluridine32 in tRNAPro(CGG)
-
-
-
?
S-adenosyl-L-methionine + uridine32 in tRNAPro(UGG)
S-adenosyl-L-homocysteine + 2'-O-methyluridine32 in tRNAPro(UGG)
-
-
-
?
S-adenosyl-L-methionine + adenine32 in tRNAPro(GGG)
S-adenosyl-L-homocysteine + 2'-O-methyladenine32 in tRNAPro(GGG)
tRNAPro(GGG) is the most efficient substrate, with methyladenine formation an order of magnitude higher than methyluridine or methylcytidine in other substrates
-
-
?
S-adenosyl-L-methionine + adenine32 in tRNAPro(GGG)
S-adenosyl-L-homocysteine + 2'-O-methyladenine32 in tRNAPro(GGG)
tRNAPro(GGG) is the most efficient substrate, with methyladenine formation an order of magnitude higher than methyluridine or methylcytidine in other substrates
-
-
?
S-adenosyl-L-methionine + cytidine32 in tRNA
S-adenosyl-L-homocysteine + 2'-O-methylcytidine32 in tRNA
presence of 2'-O-methylated cytidine at position 32 in tRNAfMet1(CAU), tRNAfMet2(CAU), tRNASer1(UGA), and tRNATrp1(CCA). In Escherichia coli YfhQ is the only methyltransferase responsible for the formation of Cm32 in tRNA
-
-
?
S-adenosyl-L-methionine + cytidine32 in tRNA
S-adenosyl-L-homocysteine + 2'-O-methylcytidine32 in tRNA
2'-O-methylation of cytidine34 in tRNAGln2(CUG). Total crude tRNA extracted from the wild-type strain MC1061 was not a substrate for the purified YfhQ enzyme, while tRNA from the yfhQ K.O. strain is an excellent substrate for this enzyme
-
-
?
S-adenosyl-L-methionine + cytidine32 in tRNA
S-adenosyl-L-homocysteine + 2'-O-methylcytidine32 in tRNA
-
-
-
?
S-adenosyl-L-methionine + cytidine32 in tRNA
S-adenosyl-L-homocysteine + 2'-O-methylcytidine32 in tRNA
-
-
-
?
S-adenosyl-L-methionine + cytidine32 in tRNA
S-adenosyl-L-homocysteine + 2'-O-methylcytidine32 in tRNA
while the four canonical nucleosides are substrates of the Escherichia coli enzyme at position 32 of tRNA, the archaeal TrmJ can only methylate the ribose of a cytidine
-
-
?
S-adenosyl-L-methionine + cytidine32 in tRNA
S-adenosyl-L-homocysteine + 2'-O-methylcytidine32 in tRNA
while the four canonical nucleosides are substrates of the Escherichia coli enzyme at position 32 of tRNA, the archaeal TrmJ can only methylate the ribose of a cytidine
-
-
?
S-adenosyl-L-methionine + cytidine32 in tRNAMet(CAU)
S-adenosyl-L-homocysteine + 2'-O-methylcytisine32 in tRNA
-
-
-
?
S-adenosyl-L-methionine + cytidine32 in tRNAMet(CAU)
S-adenosyl-L-homocysteine + 2'-O-methylcytisine32 in tRNA
-
-
-
?
S-adenosyl-L-methionine + cytidine32 in tRNATrp(CCA)
S-adenosyl-L-homocysteine + 2'-O-methylcytidine32 in tRNATrp(CCA)
-
-
-
?
S-adenosyl-L-methionine + cytidine32 in tRNATrp(CCA)
S-adenosyl-L-homocysteine + 2'-O-methylcytidine32 in tRNATrp(CCA)
-
-
-
?
S-adenosyl-L-methionine + nucleoside32 in EctRNAfMet1(CAU)
S-adenosyl-L-homocysteine + 2'-O-methylnucleoside32 in EctRNAfMet1(CAU)
-
-
-
?
S-adenosyl-L-methionine + nucleoside32 in EctRNAfMet1(CAU)
S-adenosyl-L-homocysteine + 2'-O-methylnucleoside32 in EctRNAfMet1(CAU)
-
-
-
?
S-adenosyl-L-methionine + nucleoside32 in EctRNAfMet2(CAU)
S-adenosyl-L-homocysteine + 2'-O-methylnucleoside32 in EctRNAfMet2(CAU)
-
-
-
?
S-adenosyl-L-methionine + nucleoside32 in EctRNAfMet2(CAU)
S-adenosyl-L-homocysteine + 2'-O-methylnucleoside32 in EctRNAfMet2(CAU)
-
-
-
?
S-adenosyl-L-methionine + nucleoside32 in EctRNAGln1(UUG)
S-adenosyl-L-homocysteine + 2'-O-methylnucleoside32 in EctRNAGln1(UUG)
-
-
-
?
S-adenosyl-L-methionine + nucleoside32 in EctRNAGln1(UUG)
S-adenosyl-L-homocysteine + 2'-O-methylnucleoside32 in EctRNAGln1(UUG)
-
-
-
?
S-adenosyl-L-methionine + nucleoside32 in tRNA
S-adenosyl-L-homocysteine + 2'-O-methylnucleoside32 in tRNA
-
-
-
?
S-adenosyl-L-methionine + nucleoside32 in tRNA
S-adenosyl-L-homocysteine + 2'-O-methylnucleoside32 in tRNA
-
-
-
?
S-adenosyl-L-methionine + uridine32 in tRNA
S-adenosyl-L-homocysteine + 2'-O-methyluridine32 in tRNA
presence of 2'-O-methylated uridine in position 32 of the anticodon loop in tRNAGln1(UUG) and tRNAGln2(CUG)
-
-
?
S-adenosyl-L-methionine + uridine32 in tRNA
S-adenosyl-L-homocysteine + 2'-O-methyluridine32 in tRNA
2'-O-methylation of uridine34 tRNASer1(UGA). Total crude tRNA extracted from the wild-type strain MC1061 was not a substrate for the purified YfhQ enzyme, while tRNA from the yfhQ K.O. strain is an excellent substrate for this enzyme
-
-
?
additional information
?
-
no methylation of cytosine 34 in tRNALeu(CAA)
-
-
?
additional information
?
-
-
no methylation of cytosine 34 in tRNALeu(CAA)
-
-
?
additional information
?
-
bacterial TrmJs recognize substrate tRNAs and specifically catalyze a 2'-O modification at ribose 32. All six Escherichia coli tRNAs with 2'-O-methylated nucleosides at position 32 are substrates of EcTrmJ. The elbow region of tRNA, but not the amino acid acceptor stem, is needed for the methylation reaction. tRNA recognition by EcTrmJ involves the cooperative influences of conserved residues from both the SPOUT and extensional domains, and this process is regulated by the flexible hinge region that connects these two domains
-
-
?
additional information
?
-
bacterial TrmJs recognize substrate tRNAs and specifically catalyze a 2'-O modification at ribose 32. All six Escherichia coli tRNAs with 2'-O-methylated nucleosides at position 32 are substrates of EcTrmJ. The elbow region of tRNA, but not the amino acid acceptor stem, is needed for the methylation reaction. tRNA recognition by EcTrmJ involves the cooperative influences of conserved residues from both the SPOUT and extensional domains, and this process is regulated by the flexible hinge region that connects these two domains
-
-
?
additional information
?
-
PA14 TrmJ catalyzes 2'-O-methylation of C, U and A at position 32 in the tRNA anticodon loop. tRNA substrates for reaction with PA14 TrmJ are prepared by in vitro T7 transcription. Substrate specificity, overview. No or poor activity with tRNASer(UGA) and tRNAHis(GUG). Structure analysis of TrmJ-NTD tRNA binding
-
-
-
additional information
?
-
PA14 TrmJ catalyzes 2'-O-methylation of C, U and A at position 32 in the tRNA anticodon loop. tRNA substrates for reaction with PA14 TrmJ are prepared by in vitro T7 transcription. Substrate specificity, overview. No or poor activity with tRNASer(UGA) and tRNAHis(GUG). Structure analysis of TrmJ-NTD tRNA binding
-
-
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