2.1.1.200: tRNA (cytidine32/uridine32-2'-O)-methyltransferase

This is an abbreviated version!
For detailed information about tRNA (cytidine32/uridine32-2'-O)-methyltransferase, go to the full flat file.

Reaction

Synonyms

2'-O-tRNA methyltransferase, PA14 14690, Saci_0621, TrMet(Xm32), TrmJ, tRNA (cytidine(32)/uridine(32)/adenosine(32)-2'-O)-methyltransferase, tRNA:Cm32/Um32 methyltransferase, tRNA:Cm32/Um32/Am32 methyltransferase, YfhQ

ECTree

     2 Transferases

         2.1 Transferring one-carbon groups

             2.1.1 Methyltransferases

                2.1.1.200 tRNA (cytidine32/uridine32-2'-O)-methyltransferase

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Results	in table
14870	AA Sequence
3	Cloned(Commentary)
2	Cofactor
3	Crystallization (Commentary)
14	General Information
1	Inhibitors
6	KM Value [mM]
2	Metals/Ions
3	Molecular Weight [Da]
8	Natural Substrates/ Products (Substrates)
7	Organism
21	PDB ID
2	pH Optimum
11	Protein Variants
3	Purification (Commentary)
2	Reaction
7	Reference
33	Substrates and Products (Substrate)
8	Subunits
19	Synonyms
1	Systematic Name
2	Temperature Optimum [°C]
6	Turnover Number [1/s]

Engineering

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Engineering on EC 2.1.1.200 - tRNA (cytidine32/uridine32-2'-O)-methyltransferase

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PROTEIN VARIANTS

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

E225A

Escherichia coli

P0AE01

site-directed mutagenesis, inactive mutant

736907

F199A

Escherichia coli

P0AE01

site-directed mutagenesis, the mutant shows highly reduced activity compared to the wild-type enzyme

736907

G231A

Escherichia coli

P0AE01

site-directed mutagenesis, inactive mutant

736907

I228A

Escherichia coli

P0AE01

site-directed mutagenesis, the mutant shows highly reduced activity compared to the wild-type enzyme

736907

L229A

Escherichia coli

P0AE01

site-directed mutagenesis, inactive mutant

736907

E225A

Escherichia coli K-12 MG1655

-

site-directed mutagenesis, inactive mutant

-

F199A

Escherichia coli K-12 MG1655

-

site-directed mutagenesis, the mutant shows highly reduced activity compared to the wild-type enzyme

-

G231A

Escherichia coli K-12 MG1655

-

site-directed mutagenesis, inactive mutant

-

L229A

Escherichia coli K-12 MG1655

-

site-directed mutagenesis, inactive mutant

-

additional information

2 entries

additional information

Escherichia coli

P0AE01

generation of several partial deletion mutants of TrmJ, including single site mutants, DELTA2 (deleted residues 171-172), DELTA4 (deleted residues 171-174), DELTA7 (deleted residues 169-175), DELTA10 (deleted residues 167-176), DELTA12 (deleted residues 166-177), NTD (residues 1-170) and CTD (residues 171-246)

736907

additional information

Escherichia coli K-12 MG1655

-

generation of several partial deletion mutants of TrmJ, including single site mutants, DELTA2 (deleted residues 171-172), DELTA4 (deleted residues 171-174), DELTA7 (deleted residues 169-175), DELTA10 (deleted residues 167-176), DELTA12 (deleted residues 166-177), NTD (residues 1-170) and CTD (residues 171-246)

-