2.1.1.199: 16S rRNA (cytosine1402-N4)-methyltransferase
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For detailed information about 16S rRNA (cytosine1402-N4)-methyltransferase, go to the full flat file.
Reaction
Synonyms
methyltransferase RsmH, mraW, RsmH
ECTree
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Crystallization
Crystallization on EC 2.1.1.199 - 16S rRNA (cytosine1402-N4)-methyltransferase
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purified recombinant His6-tagged RsmH in complex with S-adenosyl-L-methionine and cytidine in a 1:5:5 ratio, sitting drop vapour diffusion method, from 20 mM Tris, 100 mM NaCl, 5% v/v glycerol, 2 mM DTT, pH 8.0, room temperature, the best crystal is obtained in the mixture solution 1.0 M ammonium sulfate, 0.1 M HEPES, pH 6.5, and 0.5% w/v PEG 8, 000 in 3-4 days, X-ray diffraction structure determination and analysis at 2.25 A resolution