2.1.1.197: malonyl-[acyl-carrier protein] O-methyltransferase

This is an abbreviated version!
For detailed information about malonyl-[acyl-carrier protein] O-methyltransferase, go to the full flat file.

Reaction

Synonyms

BioC, S-adenosyl-L-methionine-dependent methyltransferase, SAM-dependent methyltransferase

ECTree

     2 Transferases

         2.1 Transferring one-carbon groups

             2.1.1 Methyltransferases

                2.1.1.197 malonyl-[acyl-carrier protein] O-methyltransferase

Advanced search results

Do not include text mining results

Include results (more...)

Include results (more...)

Results	in table
10145	AA Sequence
2	Cloned(Commentary)
1	Cofactor
1	Expression
5	General Information
3	Inhibitors
2	kcat/KM [mM/s]
2	KM Value [mM]
1	Metals/Ions
1	Molecular Weight [Da]
3	Natural Substrates/ Products (Substrates)
8	Organism
3	Pathway
1	pH Optimum
1	pH Range
1	pI Value
4	Protein Variants
1	Purification (Commentary)
1	Reaction
7	Reference
10	Substrates and Products (Substrate)
1	Subunits
4	Synonyms
1	Systematic Name
2	Turnover Number [1/s]

General Information

print visible entries

print all entries

Go back to the abbreviated version
of the Enzyme Summary Page.

General Information on EC 2.1.1.197 - malonyl-[acyl-carrier protein] O-methyltransferase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.

top print hide show all columns Go to General Information Search

Please wait a moment until the data is sorted. This message will disappear when the data is sorted.

GENERAL INFORMATION

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

malfunction

Escherichia coli

-

the DELTAbioC deletion mutant is only able to grow in biotin supplemented medium. Also supplementation with pimelic acid, and putative intermediates in the pathway, the enoyl, 3-keto and 3-hydroxy derivatives of the monomethyl ester of glutarate and the 3-keto and 3-hydroxy derivatives of the monomethyl ester of pimelate, allow growth of the mutant strain in the absence of biotin, but the 2-keto, 2-hydroxy and 4-keto derivatives, as well as monomethyl esters of C4, C6, C8, C9 and C11 dicarboxylates, do not, overview

710038

metabolism

Escherichia coli

-

the pimeloyl moiety of biotin is synthesized by a modified fatty acid synthetic pathway in which the omega-carboxyl group of a malonyl-thioester is methylated by BioC which allows recognition of this atypical substrate by the fatty acid synthetic enzymes. The malonyl-thioester methyl ester enters fatty acid synthesis as the primer and undergoes two reiterations of the fatty acid elongation cycle to give pimeloyl-acyl carrier protein methyl ester which is hydrolyzed to pimeloyl-ACP and methanol by BioH

710038

physiological function

3 entries

physiological function

Escherichia coli

-

deletion and complementation analysis of the biotin gene cluster. Mutants in BioC are blocked early in the biosynthetic pathway and complement mutants in bioA, bioB, and bioD

708906

physiological function

Escherichia coli

-

in biotin synthesis, the pimeloyl moiety is synthesized by a modified fatty acid synthetic pathway in which the omega-carboxyl group of a malonyl-thioester is methylated by BioC, which allows recognition of this atypical substrate by the fatty acid synthetic enzymes. The malonyl-thioester methyl ester enters fatty acid synthesis as the primer and undergoes two reiterations of the fatty acid elongation cycle to give pimeloyl-acyl carrier protein methyl ester. Supplementation of biotin-free medium with any of malonic, glutaric and pimelic acid monomethyl ester allows for acyl-ACP-synthetase-dependent growth of the bioC deletion strain

710038

physiological function

Escherichia coli

-

the role of BioC is to convert the free carboxyl group of a malonylthioester to its methyl ester by transfer of a methyl group from SAM. Methylation both cancels the charge of the carboxyl group and provides a methyl carbon to mimic the methyl ends of normal fatty acyl chains

710038