2.1.1.192: 23S rRNA (adenine2503-C2)-methyltransferase
This is an abbreviated version!
For detailed information about 23S rRNA (adenine2503-C2)-methyltransferase, go to the full flat file.
Word Map on EC 2.1.1.192
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2.1.1.192
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5\'-deoxyadenosyl
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5'-deoxyadenosine
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radical-mediated
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ripps
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desii
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venezuelae
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maturase
- 2.1.1.192
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5\'-deoxyadenosyl
- 5'-deoxyadenosine
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radical-mediated
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ripps
- desii
- venezuelae
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maturase
Reaction
2 S-adenosyl-L-methionine
+
Synonyms
Cfr, EC 2.1.1.194, methyltransferase YfgB/RlmN, NlmA, radical AdoMet rRNA methyltransferase, radical S-adenosyl-L-methionine enzyme, radical SAM enzyme, RlmN, RlmN methyltransferase, YfgB, YfgB/RlmN
ECTree
2.1.1.192 23S rRNA (adenine2503-C2)-methyltransferaseAdvanced search results
results (
results (Substrates Products
Substrates Products on EC 2.1.1.192 - 23S rRNA (adenine2503-C2)-methyltransferase
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SUBSTRATE 
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
2 S-adenosyl-L-methionine + adenine in 155mer RNA
S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 2-methyladenine in 155mer RNA
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-
-
-
?
2 S-adenosyl-L-methionine + adenine2503 in 23S rRNA
S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 2-methyladenine2503 in 23S rRNA
2 S-adenosyl-L-methionine + adenine2503 in 23S rRNA + 2 reduced [2Fe-2S] ferredoxin
S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 2-methyladenine2503 in 23S rRNA + 2 oxidized [2Fe-2S] ferredoxin
2 S-adenosyl-L-methionine + adenine2503 in 23S rRNA + 2 reduced [2Fe-2S] ferredoxin
S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 8-methyladenine2503 in 23S rRNA + 2 oxidized [2Fe-2S] ferredoxin
2 S-adenosyl-L-methionine + adenine2503 in 23S rRNA + 2 reduced [4Fe-4S] ferredoxin
S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 2-methyladenine2503 in 23S rRNA + 2 oxidized [4Fe-4S] ferredoxin
2 S-adenosyl-L-methionine + adenine37 in tRNA + 2 reduced [2Fe-2S] ferredoxin
2 S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 2-methyladenine37 in tRNA + 2 oxidized [2Fe-2S] ferredoxin
2 S-adenosyl-L-methionine + adenine37 in tRNAArg(ACG) + 2 reduced [2Fe-2S] ferredoxin
2 S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 2-methyladenine37 in tRNAArg(ACG) + 2 oxidized [2Fe-2S] ferredoxin
low activity with tRNAArg(ACG)
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-
?
2 S-adenosyl-L-methionine + adenine37 in tRNAAsp(GUC) + 2 reduced [2Fe-2S] ferredoxin
2 S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 2-methyladenine37 in tRNAAsp(GUC) + 2 oxidized [2Fe-2S] ferredoxin
very low activity with tRNAAsp(GUC)
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-
?
2 S-adenosyl-L-methionine + adenine37 in tRNAGln(CUG) + 2 reduced [2Fe-2S] ferredoxin
2 S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 2-methyladenine37 in tRNAGln(CUG) + 2 oxidized [2Fe-2S] ferredoxin
moderate activity with tRNAGln(CUG)
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-
?
2 S-adenosyl-L-methionine + adenine37 in tRNAGln(UUG) + 2 reduced [2Fe-2S] ferredoxin
2 S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 2-methyladenine37 in tRNAGln(UUG) + 2 oxidized [2Fe-2S] ferredoxin
high activity with tRNAGln(UUG)
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-
?
2 S-adenosyl-L-methionine + adenine37 in tRNAGlu(UUC) + 2 reduced [2Fe-2S] ferredoxin
2 S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 2-methyladenine37 in tRNAGlu(UUC) + 2 oxidized [2Fe-2S] ferredoxin
low activity with tRNAGlu(UUC)
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-
?
2 S-adenosyl-L-methionine + adenine37 in tRNAHis(GUG) + 2 reduced [2Fe-2S] ferredoxin
2 S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 2-methyladenine37 in tRNAHis(GUG) + 2 oxidized [2Fe-2S] ferredoxin
best tRNA substrate
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-
?
S-adenosyl-L-methionine + adenine2503 in 23S rRNA
S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 2-methyladenine2503 in 23S rRNA
2 S-adenosyl-L-methionine + adenine2503 in 23S rRNA
S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 2-methyladenine2503 in 23S rRNA
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-
-
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?
2 S-adenosyl-L-methionine + adenine2503 in 23S rRNA
S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 2-methyladenine2503 in 23S rRNA
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the enzyme catalyzes the natural post-transcriptional modification of A2503 to m2A in Escherichia coli 23S rRNA
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-
?
2 S-adenosyl-L-methionine + adenine2503 in 23S rRNA
S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 2-methyladenine2503 in 23S rRNA
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-
-
?
2 S-adenosyl-L-methionine + adenine2503 in 23S rRNA
S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 2-methyladenine2503 in 23S rRNA
RlmN is an endogenous enzyme used by the cell to refine functions of the ribosome in protein synthesis
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-
?
2 S-adenosyl-L-methionine + adenine2503 in 23S rRNA
S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 2-methyladenine2503 in 23S rRNA
S-adenosyl-L-methionine is both the methyl donor and the source of a 5'-deoxyadenosyl radical, which activates the substrate for methylation. The enzyme can utilize protein-free 23S rRNA as a substrate, but not the fully assembled large ribosomal subunit, suggesting that the methylations take place during the assembly of the ribosome. The key recognition elements in the 23S rRNA are helices 90-92 and the adjacent single stranded RNA that encompasses A2503
identification of the reaction product as 2-methyladenine2503
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?
2 S-adenosyl-L-methionine + adenine2503 in 23S rRNA
S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 2-methyladenine2503 in 23S rRNA
-
-
-
-
?
2 S-adenosyl-L-methionine + adenine2503 in 23S rRNA + 2 reduced [2Fe-2S] ferredoxin
S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 2-methyladenine2503 in 23S rRNA + 2 oxidized [2Fe-2S] ferredoxin
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-
-
?
2 S-adenosyl-L-methionine + adenine2503 in 23S rRNA + 2 reduced [2Fe-2S] ferredoxin
S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 2-methyladenine2503 in 23S rRNA + 2 oxidized [2Fe-2S] ferredoxin
-
-
-
-
?
2 S-adenosyl-L-methionine + adenine2503 in 23S rRNA + 2 reduced [2Fe-2S] ferredoxin
S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 2-methyladenine2503 in 23S rRNA + 2 oxidized [2Fe-2S] ferredoxin
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two S-adenosyl-L-methionine molecules serve as the cofactor by providing the 5'-deoxyadenosyl radical for substrate activation and the methyl
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?
2 S-adenosyl-L-methionine + adenine2503 in 23S rRNA + 2 reduced [2Fe-2S] ferredoxin
S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 8-methyladenine2503 in 23S rRNA + 2 oxidized [2Fe-2S] ferredoxin
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-
-
?
2 S-adenosyl-L-methionine + adenine2503 in 23S rRNA + 2 reduced [2Fe-2S] ferredoxin
S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 8-methyladenine2503 in 23S rRNA + 2 oxidized [2Fe-2S] ferredoxin
-
-
-
?
2 S-adenosyl-L-methionine + adenine2503 in 23S rRNA + 2 reduced [4Fe-4S] ferredoxin
S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 2-methyladenine2503 in 23S rRNA + 2 oxidized [4Fe-4S] ferredoxin
-
-
-
?
2 S-adenosyl-L-methionine + adenine2503 in 23S rRNA + 2 reduced [4Fe-4S] ferredoxin
S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 2-methyladenine2503 in 23S rRNA + 2 oxidized [4Fe-4S] ferredoxin
-
-
-
?
2 S-adenosyl-L-methionine + adenine2503 in 23S rRNA + 2 reduced [4Fe-4S] ferredoxin
S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 2-methyladenine2503 in 23S rRNA + 2 oxidized [4Fe-4S] ferredoxin
-
-
-
?
2 S-adenosyl-L-methionine + adenine2503 in 23S rRNA + 2 reduced [4Fe-4S] ferredoxin
S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 2-methyladenine2503 in 23S rRNA + 2 oxidized [4Fe-4S] ferredoxin
Thermus thermophilus HB8 / ATCC 27634 / DSM 579
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-
?
2 S-adenosyl-L-methionine + adenine37 in tRNA + 2 reduced [2Fe-2S] ferredoxin
2 S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 2-methyladenine37 in tRNA + 2 oxidized [2Fe-2S] ferredoxin
-
-
-
?
2 S-adenosyl-L-methionine + adenine37 in tRNA + 2 reduced [2Fe-2S] ferredoxin
2 S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 2-methyladenine37 in tRNA + 2 oxidized [2Fe-2S] ferredoxin
-
-
-
?
S-adenosyl-L-methionine + adenine2503 in 23S rRNA
S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 2-methyladenine2503 in 23S rRNA
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the enzyme only catalyzes C2 methylation of adenine2503 in 23S rRNA
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-
?
S-adenosyl-L-methionine + adenine2503 in 23S rRNA
S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 2-methyladenine2503 in 23S rRNA
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the enzyme primarily methylates C-8 in A2503 of 23S rRNA
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?
S-adenosyl-L-methionine + adenine2503 in 23S rRNA
S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 2-methyladenine2503 in 23S rRNA
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-
-
?
S-adenosyl-L-methionine + adenine2503 in 23S rRNA
S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 2-methyladenine2503 in 23S rRNA
the enzyme primarily methylates C-8 in A2503 of 23S rRNA
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-
?
S-adenosyl-L-methionine + adenine2503 in 23S rRNA
S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 2-methyladenine2503 in 23S rRNA
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the enzyme methylates C8 in adenine2503 in 23S rRNA
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?
additional information
?
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RlmN introduces m2A at position 2503 in the peptidyl transferase center of 23S RNA. RlmN is a dual-specificity enzyme that catalyzes methylation of both rRNA and tRNA, see for EC 2.1.1.66, substrate specificity analysis, overview
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?
additional information
?
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recombinant His-RlmN protein is able to catalyze the synthesis of m2A in a SAM-dependent manner on tRNAchimera UUG purified from DELTArlmN cells
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?
additional information
?
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enzyme additionally methylates adenine37 in tRNA such as EScherichia coli tRNAArgACG, tRNAAspGUC, tRNAGlnCUG, tRNAGlnUUG, tRNAGluUUC, and tRNAHisGUG. tRNA methylation requirements are consistent with radical SAM reactivity
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additional information
?
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enzyme additionally methylates adenine37 in tRNA such as EScherichia coli tRNAArgACG, tRNAAspGUC, tRNAGlnCUG, tRNAGlnUUG, tRNAGluUUC, and tRNAHisGUG. tRNA methylation requirements are consistent with radical SAM reactivity
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additional information
?
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only a subset of tRNAs that contain an adenosine at position 37 are substrates for RlmN, structural comparison of substrates and non-substrates, and generation of chimeric tRNA substrates, substrate specificity, detailed overview. No activity with tRNAGly(CCC), in vitro methylation of chimeric constructs
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additional information
?
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only a subset of tRNAs that contain an adenosine at position 37 are substrates for RlmN, structural comparison of substrates and non-substrates, and generation of chimeric tRNA substrates, substrate specificity, detailed overview. No activity with tRNAGly(CCC), in vitro methylation of chimeric constructs
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additional information
?
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Cfr methylates A2503 of 23S rRNA at C8 and C2, while RlmN only performs a C2 methylation using the same mechanism of function
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additional information
?
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methylation at C2 is similar to that at C8, cf. EC 2.1.1.224
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?
