2.1.1.186: 23S rRNA (cytidine2498-2'-O)-methyltransferase
This is an abbreviated version!
For detailed information about 23S rRNA (cytidine2498-2'-O)-methyltransferase, go to the full flat file.
Reaction
Synonyms
2O-ribose methyltransferase, Cm2498, RlmM, rRNA large subunit methyltransferase M, ygdE
ECTree
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Crystallization
Crystallization on EC 2.1.1.186 - 23S rRNA (cytidine2498-2'-O)-methyltransferase
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in complex with S-adenosyl-L-methionine, sitting drop vapor diffusion method, using 16% (w/v) polyethylene glycol 3350, 0.05 M citric acid, 0.05 M bis-tris propane pH 5.0
purified recombinant His-tagged RlmM free or in complex with S-adenosyl-L-methionine, sitting drop vapor diffusion method, 0.0015 ml 10 mg/ml protein solution os mixed with 0.0015 ml of reservoir solution containing 0.3 M ammonium tartrate, pH 7.0, and 20% PEG 3350, 20°C, 1 week, two crystal forms, X-ray diffraction structure determination and analysis at 1.9 A and 2.6 A resolution, respectively, molecular replacement