2.1.1.182: 16S rRNA (adenine1518-N6/adenine1519-N6)-dimethyltransferase

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For detailed information about 16S rRNA (adenine1518-N6/adenine1519-N6)-dimethyltransferase, go to the full flat file.

Reaction

4 S-adenosyl-L-methionine +

Synonyms

16S rRNA adenine dimethyltransferase, EC 2.1.1.48, KsgA, ksgA methyltransferase, RsmA, S-adenosylmethionine-6-N',N'-adenosyl (rRNA) dimethyltransferase

ECTree

     2 Transferases

         2.1 Transferring one-carbon groups

             2.1.1 Methyltransferases

                2.1.1.182 16S rRNA (adenine1518-N6/adenine1519-N6)-dimethyltransferase

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Results	in table
38645	AA Sequence
3	Application
11	Cloned(Commentary)
1	Cofactor
3	Crystallization (Commentary)
25	General Information
1	IC50 Value
2	Inhibitors
1	KM Value [mM]
6	Metals/Ions
4	Molecular Weight [Da]
7	Natural Substrates/ Products (Substrates)
264	Organism
29	PDB ID
3	pH Optimum
1	pH Range
8	Protein Variants
8	Purification (Commentary)
2	Reaction
30	Reference
19	Substrates and Products (Substrate)
4	Subunits
26	Synonyms
1	Systematic Name
2	Temperature Optimum [°C]

Engineering

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Engineering on EC 2.1.1.182 - 16S rRNA (adenine1518-N6/adenine1519-N6)-dimethyltransferase

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PROTEIN VARIANTS

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

C168A

Escherichia coli

-

site-directed mutagenesis, the mutation does not affect KsgA activity

718932

E43A

Escherichia coli

-

mutation located in the S-adenosylmethionine-binding motifs severely reduces methyltransferase activity, the mutation retains the ability to suppress the growth defect of the Era(E200K) strain at a low temperature

704274

E66A

2 entries

G47A

Escherichia coli

-

mutation located in the S-adenosylmethionine-binding motifs severely reduces methyltransferase activity, the mutation retains the ability to suppress the growth defect of the Era(E200K) strain at a low temperature

704274

L114P

Escherichia coli

-

site-directed mutagenesis of the active site residue, the KsgA mutant shows diminished overall activity, and impaired ability to methylate the N6-methyladenosine intermediate to produce N6,N6-dimethyladenosine. Reduced activity is not due to disruption of 30S substrate binding

718932

N113A

Escherichia coli

-

site-directed mutagenesis of the active site residue, the KsgA mutant shows diminishes activity to a level comparable to L114P without affecting the methylation of N6-methyladenosine. Reduced activity is not due to disruption of 30S substrate binding

718932

R248A

Escherichia coli

-

mutation at the C-terminal does not affect the methyltransferase activity and fails to suppress the growth defect of the Era(E200K) strain

704274

E66A

Escherichia coli

P06992

catalytically inactive enzyme form

705774

E66A

Escherichia coli

-

mutation located in the S-adenosylmethionine-binding motifs severely reduces methyltransferase activity, the mutation retains the ability to suppress the growth defect of the Era(E200K) strain at a low temperature

704274