2.1.1.178: 16S rRNA (cytosine1407-C5)-methyltransferase

This is an abbreviated version!
For detailed information about 16S rRNA (cytosine1407-C5)-methyltransferase, go to the full flat file.

Reaction

Synonyms

16S rRNA methyltransferase, housekeeping methyltransferase RsmF, RNA m5C methyltransferase YebU, RsmF, RsmF housekeeping methyltransferase, YebU

ECTree

     2 Transferases

         2.1 Transferring one-carbon groups

             2.1.1 Methyltransferases

                2.1.1.178 16S rRNA (cytosine1407-C5)-methyltransferase

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Results	in table
3252	AA Sequence
4	Cloned(Commentary)
2	Cofactor
2	Crystallization (Commentary)
10	General Information
3	Inhibitors
1	Molecular Weight [Da]
11	Natural Substrates/ Products (Substrates)
42	Organism
1	pH Optimum
4	Protein Variants
2	Purification (Commentary)
2	Reaction
9	Reference
22	Substrates and Products (Substrate)
3	Subunits
16	Synonyms
1	Systematic Name
1	Temperature Optimum [°C]
1	Temperature Stability [°C]

Substrates Products

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Substrates Products on EC 2.1.1.178 - 16S rRNA (cytosine1407-C5)-methyltransferase

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SUBSTRATE

PRODUCT                       

REACTION DIAGRAM

ORGANISM

UNIPROT

COMMENTARY
(Substrate)

LITERATURE
(Substrate)

COMMENTARY
(Product)

LITERATURE
(Product)

Reversibility
r=reversible
ir=irreversible
?=not specified

S-adenosyl-L-methionine + cytosine1400 in 16S rRNA

S-adenosyl-L-homocysteine + 5-methylcytosine1400 in 16S rRNA

4 entries

S-adenosyl-L-methionine + cytosine1404 in 16S rRNA

S-adenosyl-L-homocysteine + 5-methylcytosine1404 in 16S rRNA

2 entries

S-adenosyl-L-methionine + cytosine1407 in 16S rRNA

S-adenosyl-L-homocysteine + 5-methylcytosine1407 in 16S rRNA

13 entries

additional information

?

-

2 entries

S-adenosyl-L-methionine + cytosine1400 in 16S rRNA

S-adenosyl-L-homocysteine + 5-methylcytosine1400 in 16S rRNA

Thermus thermophilus

Q5SJI2

-

721025

-

-

?

S-adenosyl-L-methionine + cytosine1400 in 16S rRNA

S-adenosyl-L-homocysteine + 5-methylcytosine1400 in 16S rRNA

Thermus thermophilus

Q5SJI2

RsmF completely methylates C1400 when either 16S rRNA or 30S subunits are used as a substrate

721025

-

-

?

S-adenosyl-L-methionine + cytosine1400 in 16S rRNA

S-adenosyl-L-homocysteine + 5-methylcytosine1400 in 16S rRNA

Thermus thermophilus HB8 / ATCC 27634 / DSM 579

Q5SJI2

-

721025

-

-

?

S-adenosyl-L-methionine + cytosine1400 in 16S rRNA

S-adenosyl-L-homocysteine + 5-methylcytosine1400 in 16S rRNA

Thermus thermophilus HB8 / ATCC 27634 / DSM 579

Q5SJI2

RsmF completely methylates C1400 when either 16S rRNA or 30S subunits are used as a substrate

721025

-

-

?

S-adenosyl-L-methionine + cytosine1404 in 16S rRNA

S-adenosyl-L-homocysteine + 5-methylcytosine1404 in 16S rRNA

Thermus thermophilus

Q5SJI2

-

721025

-

-

?

S-adenosyl-L-methionine + cytosine1404 in 16S rRNA

S-adenosyl-L-homocysteine + 5-methylcytosine1404 in 16S rRNA

Thermus thermophilus

Q5SJI2

RsmF methylates C1404 to about 35% with 16S rRNA and completely with 30S subunits

721025

-

-

?

S-adenosyl-L-methionine + cytosine1407 in 16S rRNA

S-adenosyl-L-homocysteine + 5-methylcytosine1407 in 16S rRNA

Escherichia coli

-

-

718596, 735241

-

-

?

S-adenosyl-L-methionine + cytosine1407 in 16S rRNA

S-adenosyl-L-homocysteine + 5-methylcytosine1407 in 16S rRNA

Escherichia coli

P76273

-

705112

-

-

?

S-adenosyl-L-methionine + cytosine1407 in 16S rRNA

S-adenosyl-L-homocysteine + 5-methylcytosine1407 in 16S rRNA

Escherichia coli

P76273

nucleotide C1407 is located at a functionally active region of the 30 S subunit interface close to the P site. YebU-directed methylation of this nucleotide seems to be conserved in bacteria

705111

-

-

?

S-adenosyl-L-methionine + cytosine1407 in 16S rRNA

S-adenosyl-L-homocysteine + 5-methylcytosine1407 in 16S rRNA

Escherichia coli

P76273

purified recombinant YebU protein retains its specificity for C1407 in vitro, and methylates 30 S subunits (but not naked 16 S rRNA or 70 S ribosomes) isolated from yebU knockout strains

705111

-

-

?

S-adenosyl-L-methionine + cytosine1407 in 16S rRNA

S-adenosyl-L-homocysteine + 5-methylcytosine1407 in 16S rRNA

Escherichia coli

-

nucleotide C1407 forms part of the aminoglycoside binding pocket of the 16S rRNA

718596

-

-

?

S-adenosyl-L-methionine + cytosine1407 in 16S rRNA

S-adenosyl-L-homocysteine + 5-methylcytosine1407 in 16S rRNA

Escherichia coli BW25113

-

-

718596

-

-

?

S-adenosyl-L-methionine + cytosine1407 in 16S rRNA

S-adenosyl-L-homocysteine + 5-methylcytosine1407 in 16S rRNA

Escherichia coli BW25113

-

nucleotide C1407 forms part of the aminoglycoside binding pocket of the 16S rRNA

718596

-

-

?

S-adenosyl-L-methionine + cytosine1407 in 16S rRNA

S-adenosyl-L-homocysteine + 5-methylcytosine1407 in 16S rRNA

Escherichia coli BW25113

-

-

735241

-

-

?

S-adenosyl-L-methionine + cytosine1407 in 16S rRNA

S-adenosyl-L-homocysteine + 5-methylcytosine1407 in 16S rRNA

Escherichia coli MM294

-

-

735241

-

-

?

S-adenosyl-L-methionine + cytosine1407 in 16S rRNA

S-adenosyl-L-homocysteine + 5-methylcytosine1407 in 16S rRNA

Thermus thermophilus

Q5SJI2

-

721025

-

-

?

S-adenosyl-L-methionine + cytosine1407 in 16S rRNA

S-adenosyl-L-homocysteine + 5-methylcytosine1407 in 16S rRNA

Thermus thermophilus

Q5SJI2

RsmF produces only trace amounts of methylation of C1407 with 16S rRNA and about 75% with 30S subunits

721025

-

-

?

S-adenosyl-L-methionine + cytosine1407 in 16S rRNA

S-adenosyl-L-homocysteine + 5-methylcytosine1407 in 16S rRNA

Thermus thermophilus HB8 / ATCC 27634 / DSM 579

Q5SJI2

-

721025

-

-

?

S-adenosyl-L-methionine + cytosine1407 in 16S rRNA

S-adenosyl-L-homocysteine + 5-methylcytosine1407 in 16S rRNA

Thermus thermophilus HB8 / ATCC 27634 / DSM 579

Q5SJI2

RsmF produces only trace amounts of methylation of C1407 with 16S rRNA and about 75% with 30S subunits

721025

-

-

?

additional information

?

-

Thermus thermophilus

Q5SJI2

substrate recognition mechanism, overview

721025

-

-

?

additional information

?

-

Thermus thermophilus HB8 / ATCC 27634 / DSM 579

Q5SJI2

substrate recognition mechanism, overview

721025

-

-

?