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2.1.1.178: 16S rRNA (cytosine1407-C5)-methyltransferase

This is an abbreviated version!
For detailed information about 16S rRNA (cytosine1407-C5)-methyltransferase, go to the full flat file.

Word Map on EC 2.1.1.178

Reaction

S-adenosyl-L-methionine
+
cytosine1407 in 16S rRNA
=
S-adenosyl-L-homocysteine
 +
5-methylcytosine1407 in 16S rRNA

Synonyms

16S rRNA methyltransferase, housekeeping methyltransferase RsmF, RNA m5C methyltransferase YebU, RsmF, RsmF housekeeping methyltransferase, YebU

ECTree

     2 Transferases
         2.1 Transferring one-carbon groups
             2.1.1 Methyltransferases
                2.1.1.178 16S rRNA (cytosine1407-C5)-methyltransferase

Cloned

Cloned on EC 2.1.1.178 - 16S rRNA (cytosine1407-C5)-methyltransferase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
gene rsmF, expression of wild-type enzyme in Escherichi coli Top10 cells
-
the Escherichia coli YebU gene is cloned into the PT73.3HisGW vector.25 The resulting construct encodes a polypeptide with the YebU gene and both N- and C-terminal hexa-histidine tails
the yebU gene is cloned as a partial and as a fulllength sequence with the respective purposes of creating a knockout strain and expressing recombinant YebU protein. Full-length YebU protein with a C-terminal histidine-tag is expressed in Escherichia coli