2.1.1.176: 16S rRNA (cytosine967-C5)-methyltransferase

This is an abbreviated version!
For detailed information about 16S rRNA (cytosine967-C5)-methyltransferase, go to the full flat file.

Reaction

Synonyms

16S rRNA m5C967 methyltransferase, 16S rRNA methyltransferase, fma, fmu, Fmu/RsmB/RrmB, Fmu/RsmB/RrmB rRNA cytosine 5-methyltransferase, m5C methyltransferase Fmu, PH0851, RNA:m5C MTase, rsmB

ECTree

     2 Transferases

         2.1 Transferring one-carbon groups

             2.1.1 Methyltransferases

                2.1.1.176 16S rRNA (cytosine967-C5)-methyltransferase

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Results	in table
19752	AA Sequence
3	Cloned(Commentary)
4	Cofactor
2	Crystallization (Commentary)
8	General Information
1	kcat/KM [mM/s]
1	KM Value [mM]
1	Metals/Ions
2	Molecular Weight [Da]
11	Natural Substrates/ Products (Substrates)
165	Organism
2	pH Optimum
2	Protein Variants
2	Purification (Commentary)
2	Reaction
12	Reference
15	Substrates and Products (Substrate)
7	Subunits
22	Synonyms
1	Systematic Name
2	Temperature Optimum [°C]
1	Turnover Number [1/s]

Substrates Products

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Substrates Products on EC 2.1.1.176 - 16S rRNA (cytosine967-C5)-methyltransferase

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SUBSTRATE

PRODUCT                       

REACTION DIAGRAM

ORGANISM

UNIPROT

COMMENTARY
(Substrate)

LITERATURE
(Substrate)

COMMENTARY
(Product)

LITERATURE
(Product)

Reversibility
r=reversible
ir=irreversible
?=not specified

S-adenosyl-L-methionine + cytosine967 in 16S rRNA

S-adenosyl-L-homocysteine + 5-methylcytosine967 in 16S rRNA

9 entries

additional information

?

-

2 entries

S-adenosyl-L-methionine + cytosine967 in 16S rRNA

S-adenosyl-L-homocysteine + 5-methylcytosine967 in 16S rRNA

Escherichia coli

-

-

718474, 734085, 734794, 735258

-

-

?

S-adenosyl-L-methionine + cytosine967 in 16S rRNA

S-adenosyl-L-homocysteine + 5-methylcytosine967 in 16S rRNA

Escherichia coli

P36929

-

702199, 702200

-

-

?

S-adenosyl-L-methionine + cytosine967 in 16S rRNA

S-adenosyl-L-homocysteine + 5-methylcytosine967 in 16S rRNA

Escherichia coli

-

-

702200

-

-

?

S-adenosyl-L-methionine + cytosine967 in 16S rRNA

S-adenosyl-L-homocysteine + 5-methylcytosine967 in 16S rRNA

Escherichia coli

P36929

-

706780

-

-

?

S-adenosyl-L-methionine + cytosine967 in 16S rRNA

S-adenosyl-L-homocysteine + 5-methylcytosine967 in 16S rRNA

Escherichia coli

P36929

the recombinant protein exhibits the same specificity as the native enzyme. It forms only m5C and only at position 967. C1407, which is also 5-methylcytosine in natural 16S RNA, is not methylated. In vitro, the enzyme only recognizes free 16S RNA. 30S ribosomal subunits are not a substrate

702199

-

-

?

S-adenosyl-L-methionine + cytosine967 in 16S rRNA

S-adenosyl-L-homocysteine + 5-methylcytosine967 in 16S rRNA

Escherichia coli BW25113

-

-

734085, 735258

-

-

?

S-adenosyl-L-methionine + cytosine967 in 16S rRNA

S-adenosyl-L-homocysteine + 5-methylcytosine967 in 16S rRNA

Pyrococcus horikoshii

O58581

-

718474

-

-

?

S-adenosyl-L-methionine + cytosine967 in 16S rRNA

S-adenosyl-L-homocysteine + 5-methylcytosine967 in 16S rRNA

Thermus thermophilus

-

-

721025

-

-

?

S-adenosyl-L-methionine + cytosine967 in 16S rRNA

S-adenosyl-L-homocysteine + 5-methylcytosine967 in 16S rRNA

Thermus thermophilus HB8 / ATCC 27634 / DSM 579

-

-

721025

-

-

?

additional information

?

-

Thermus thermophilus

-

substrate recognition mechanism, overview

721025

-

-

?

additional information

?

-

Thermus thermophilus HB8 / ATCC 27634 / DSM 579

-

substrate recognition mechanism, overview

721025

-

-

?