2.1.1.176: 16S rRNA (cytosine967-C5)-methyltransferase
This is an abbreviated version!
For detailed information about 16S rRNA (cytosine967-C5)-methyltransferase, go to the full flat file.
Reaction
Synonyms
16S rRNA m5C967 methyltransferase, 16S rRNA methyltransferase, fma, fmu, Fmu/RsmB/RrmB, Fmu/RsmB/RrmB rRNA cytosine 5-methyltransferase, m5C methyltransferase Fmu, PH0851, RNA:m5C MTase, rsmB
ECTree
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Crystallization
Crystallization on EC 2.1.1.176 - 16S rRNA (cytosine967-C5)-methyltransferase
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hanging-drop vapor diffusion method, crystal structure of Escherichia coli Fmu, determined at 1.65 A resolution for the apoenzyme and 2.1 A resolution in complex with S-adenosyl-L-methionine
purified wild-type and SeMet-labeled PH0851 complexed with S-adenosyl-L-methionine-analogue sinefungin, hanging drop vapour diffusion method, mixing of protein solution, containing 1.7 mg/ml protein in 20 mM Tris-HCl buffer, pH 8.0, 400 mM NaCl, and 1 mM sinefungin, with reservoir solution, containing 80 mM HEPES-Na buffer, pH 7.0, 1.15 M sodium citrate, and 7-15% w/v 1,6-hexanediol, in a 4:1 ratio, 20°C, 1 week, X-ray diffraction structure determination and analysis at 2.55-3.0 A resolution, molecular replacement