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2.1.1.174: 23S rRNA (guanine1835-N2)-methyltransferase

This is an abbreviated version!
For detailed information about 23S rRNA (guanine1835-N2)-methyltransferase, go to the full flat file.

Reaction

S-adenosyl-L-methionine
+
guanine1835 in 23S rRNA
=
S-adenosyl-L-homocysteine
+
N2-methylguanine1835 in 23S rRNA

Synonyms

23S rRNA methyltransferase, EC 2.1.1.51, EC 2.1.1.52, m2G1835, N2-methyltransferase RlmG, ribosomal RNA large subunit methyltransferase G, RlmG, ygjO

ECTree

     2 Transferases
         2.1 Transferring one-carbon groups
             2.1.1 Methyltransferases
                2.1.1.174 23S rRNA (guanine1835-N2)-methyltransferase

Crystallization

Crystallization on EC 2.1.1.174 - 23S rRNA (guanine1835-N2)-methyltransferase

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CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
RlmG in complex with S-adenosyl-L-methionine, sitting drop vapor diffusion method, room temperature, mixture of RlmG with S-adenosyl-L-methionine in solution 0.2 M Tris, pH 7.5, and 5% w/v PEG 8000 with the addition of 1% w/v protamine sulfate, and 0.02 M HEPES sodium, pH 6.8, 3-4 days, X-ray diffraction structure determination and analysis at 2.3 A resolution, single-wavelength anomalous dispersion