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2.1.1.171: 16S rRNA (guanine966-N2)-methyltransferase

This is an abbreviated version!
For detailed information about 16S rRNA (guanine966-N2)-methyltransferase, go to the full flat file.

Word Map on EC 2.1.1.171

Reaction

S-adenosyl-L-methionine
+
guanine966 in 16S rRNA
=
S-adenosyl-L-homocysteine
 +
N2-methylguanine966 in 16S rRNA

Synonyms

16S rRNA-specific methyltransferases m2GMT, EC 2.1.1.51, EC 2.1.1.52, m2G966 methyltransferase, RNA:(guanine-N2) methyltransferase RsmD, RsmD, RsmD-like methyltransferase, Rv2966c, Rv2966c protein, small rRNA methyltransferase, yhhF

ECTree

     2 Transferases
         2.1 Transferring one-carbon groups
             2.1.1 Methyltransferases
                2.1.1.171 16S rRNA (guanine966-N2)-methyltransferase

Crystallization

Crystallization on EC 2.1.1.171 - 16S rRNA (guanine966-N2)-methyltransferase

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CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
vapor diffusion method in hanging drops, structure determined and refined to 2.05 A
purified recombinant Rv2966c, hanging drop vapour diffusion method, mixing of 0.004 ml of 17 mg/ml Rv2966c in 10 mM potassium phosphate buffer, pH 7.4, 5 mM EDTA, 10% v/v glycerol, 0.1% v/v 2-mercaptoethanol, 50 mM NaCl, 0.1 mM S-adenosyl-L-methionine, with 0.002 ml of reservoir solution containing 100 mM Tris-HCl, pH 7.4, 3.0 M potassium acetate at 24 °C, 2-3 days, X-ray diffraction structure determination and analysis at 1.9 A resolution, molecular replacement