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2.1.1.170: 16S rRNA (guanine527-N7)-methyltransferase

This is an abbreviated version!
For detailed information about 16S rRNA (guanine527-N7)-methyltransferase, go to the full flat file.

Word Map on EC 2.1.1.170

Reaction

S-adenosyl-L-methionine
+
guanine527 in 16S rRNA
=
S-adenosyl-L-homocysteine
 +
N7-methylguanine527 in 16S rRNA

Synonyms

16S rRNA methyltransferase, 16S rRNA methyltransferase RsmG, gidB, glucose-inhibited division protein B, ribosomal RNA small subunit methyltransferase G, rsmG, RsmG methyltransferase

ECTree

     2 Transferases
         2.1 Transferring one-carbon groups
             2.1.1 Methyltransferases
                2.1.1.170 16S rRNA (guanine527-N7)-methyltransferase

Cloned

Cloned on EC 2.1.1.170 - 16S rRNA (guanine527-N7)-methyltransferase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli BL21(DE3) cells
expression in Escherichia coli BL21
gene rsmG is the second member in a bicistronic operon, rsmG also has its own promoter, RsmG expression might depend on the activity of an inverted repeated region, located between the rsmG promoter and ribosome binding site, which works as a weak transcriptional terminator. Expression of C-terminally His6-tagged and FLAG-tagged RsmG in Escherichia coli TOP10 cells