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2.1.1.165: methyl halide transferase

This is an abbreviated version!
For detailed information about methyl halide transferase, go to the full flat file.

Word Map on EC 2.1.1.165

Reaction

S-adenosyl-L-methionine
+
Iodide
=
S-adenosyl-L-homocysteine
 +
methyl iodide

Synonyms

AtHOL1, AtHOL2, AtHOL3, halide/bisulfide methyltransferase, HMT, HMT/HTMT, HOL, HTMT, MCT, methyl chloride transferase, methyl halide transferase, MHT, S-adenosyl-L-methionine: halide ion methyltransferase, S-adenosyl-L-methionine:halide/bisulfide methyltransferase, S-adenosylmethionine-dependent halide/thiol methyltransferase, SAM:halide ion methyltransferase

ECTree

     2 Transferases
         2.1 Transferring one-carbon groups
             2.1.1 Methyltransferases
                2.1.1.165 methyl halide transferase

Purification

Purification on EC 2.1.1.165 - methyl halide transferase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
gel filtration, anion exchange chromatography, and affinity chromatography on adenosine-agarose
-
native enzyme 2700fold to homogeneity by ammonium sulfate fractionation, gel filtration, adenosine affinity chromatography, and a second step of gel filtration
the difficulty of solubilization of this membrane-bound labile enzyme is the greatest obstacle to its purification
-
the purification is achieved by an 80 to 100% ammonium sulfate precipitation step followed by a high-performance liquid chromatography gel filtration step on a 60 cm by 2.15 cm preparative Bio-Sil SEC-250 column. The column is eluted with 10 mM phosphate buffer, pH 7.0
-