2.1.1.160: caffeine synthase

This is an abbreviated version!
For detailed information about caffeine synthase, go to the full flat file.

Reaction

Synonyms

1-N-methyltransferase, 1NMT, 3, 7-dimethylxanthine methyltransferase, 3,7-dimethylxanthine methyltransferase, 7-N-methylxanthine methyltransferase, caffeine synthase, caffeine synthase 1, CCS, CCS1, coffee caffeine synthase 1, CS, CtCS6, DXMT, DXMT1, N-1 methyltransferase, TCS, TCS1, tea caffeine synthase, tea caffeine synthase 1, theobromine 1-N-methyltransferase

ECTree

     2 Transferases

         2.1 Transferring one-carbon groups

             2.1.1 Methyltransferases

                2.1.1.160 caffeine synthase

Advanced search results

Do not include text mining results

Include results (more...)

Include results (more...)

Results	in table
1	Activating Compound
18	AA Sequence
9	Application
1	CAS Registry Number
19	Cloned(Commentary)
4	Cofactor
3	Crystallization (Commentary)
11	Expression
6	General Information
7	Inhibitors
5	KM Value [mM]
2	Localization
4	Metals/Ions
12	Molecular Weight [Da]
40	Natural Substrates/ Products (Substrates)
39	Organism
6	Pathway
9	pH Optimum
2	pH Range
2	pI Value
11	Protein Variants
5	Purification (Commentary)
5	Reaction
39	Reference
45	Source Tissue
5	Specific Activity [micromol/min/mg]
71	Substrates and Products (Substrate)
9	Subunits
44	Synonyms
1	Systematic Name
7	Temperature Optimum [°C]

Purification

print visible entries

print all entries

Go back to the abbreviated version
of the Enzyme Summary Page.

Purification on EC 2.1.1.160 - caffeine synthase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.

top print hide Go to Purification (commentary) Search

Please wait a moment until the data is sorted. This message will disappear when the data is sorted.

PURIFICATION (Commentary)

ORGANISM

UNIPROT

LITERATURE

native enzyme 24fold from young leaves by 3 steps of ion exchange chromatography, and gel filtration

Coffea arabica

-

670509

native enzyme 523fold from young, developing leaves by ammonium sulfate fractionation, hydroxylapatite, anion exchange, and adenosine affinity chromatography, and gel filtration to homogeneity

Camellia sinensis

-

670577

recombinant His-tagged DXMT1 from Escherichia coli strain BL21(DE3) to homogeneity by nickel affinity chromatography, cleavage of the His-tag with tobacco etch virus, TEV, protease, followed by gel filtration

Coffea canephora

A4GE70

667101

recombinant protein

Paullinia cupana var. sorbilis

A0A061DEC0

757589

using the His-tagged affinity chromatography

Coffea canephora

A4GE70

684218