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2.1.1.160: caffeine synthase

This is an abbreviated version!
For detailed information about caffeine synthase, go to the full flat file.

Word Map on EC 2.1.1.160

Reaction

S-adenosyl-L-methionine
+
1,7-dimethylxanthine
=
S-adenosyl-L-homocysteine
 +
1,3,7-Trimethylxanthine

Synonyms

1-N-methyltransferase, 1NMT, 3, 7-dimethylxanthine methyltransferase, 3,7-dimethylxanthine methyltransferase, 7-N-methylxanthine methyltransferase, caffeine synthase, caffeine synthase 1, CCS, CCS1, coffee caffeine synthase 1, CS, CtCS6, DXMT, DXMT1, N-1 methyltransferase, TCS, TCS1, tea caffeine synthase, tea caffeine synthase 1, theobromine 1-N-methyltransferase

ECTree

     2 Transferases
         2.1 Transferring one-carbon groups
             2.1.1 Methyltransferases
                2.1.1.160 caffeine synthase

Cloned

Cloned on EC 2.1.1.160 - caffeine synthase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
Agrobacterium tumefaciens-mediated silencing of caffeine synthesis through root transformation in Camellia sinensis suign the RNAi construct, pFGC1008-CS, method, overview
B9VI89
DNA and amino acid sequence determination and analysis, expression in Escherichia coli as wild-type and as Trx-fusion protein
DNA and amino acid sequence determination and analysis, expression of wild-type and chimeric mutant enzymes in Escherichia coli strain BL21(DE3), phylogenetic analysis
expressed in Escherichia coli BL21(DE3) cells
K9MNV5
expressed in Escherichia coli Rosetta BL21 (DE3) and Saccharomyces cerevisiae INVSC1 cells
expression analysis
-
expression in Escherichia coli
expression in Escherichia coli as His-tagged fusion protein
expression of His-tagged DXMT1 in Escherichia coli strain BL21(DE3)
expression of wild-type and mutant enzymes in Escherichia coli strain BL21 (DE3), sequence comparisonof caffeine synthetic enzymes from coffee
Coffea sp.
-
gene CaMTL1, cDNA library construction, DNA and amino acid sequence determination and analysis, sequence comparison with other species
gene DXMT1, DNA and amino acid sequence determination and analysis, expression analysis
gene TCS1, cDNA and amino acid sequence determination and analysis, expression in Escherichia coli
isozyme 1, DNA and amino acid sequence determination and analysis, expression of wild-type and chimeric mutant enzymes in Escherichia coli strain BL21(DE3), phylogenetic analysis
isozyme DXMT1, DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression of the GST-tagged enzyme in Escherichia coli Bl21(DE3)
isozyme DXMT1, functional expression in transgenic Nicotiana tabacum plant leaves
semiquantitative-PCR for caffeine synthase transcript expression analysis
-