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2.1.1.158: 7-methylxanthosine synthase

This is an abbreviated version!
For detailed information about 7-methylxanthosine synthase, go to the full flat file.

Word Map on EC 2.1.1.158

Reaction

S-adenosyl-L-methionine
+
Xanthosine
=
S-adenosyl-L-homocysteine
+
7-methylxanthosine

Synonyms

7-methylxanthine 3-N-methyltransferase, 7-methylxanthine synthase, 7-methylxanthosine synthase, 7-methylxanthosine synthase 1, 7-N-methyltransferase, 7NMT, CmXRS1, N-methyltransferase, SAM:xanthosine 7-N-methyltransferase, xanthosine methyltransferase, xanthosine-N7-methyltransferase, xanthosine:S-adenosyl-L-methionine methyltransferase, XMT, XMT1, XRS1

ECTree

     2 Transferases
         2.1 Transferring one-carbon groups
             2.1.1 Methyltransferases
                2.1.1.158 7-methylxanthosine synthase

Purification

Purification on EC 2.1.1.158 - 7-methylxanthosine synthase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
native enzyme
-
native enzyme 9fold from young leaves by 3 steps of ion exchange chromatography, and gel filtration
-
native enzyme partially from young leaves by ammonium sulfate fractionation, gel filtration, and ultrafiltration
-
recombinant His-tagged XMT1 from Escherichia coli strain BL21(DE3) to homogeneity by nickel affinity chromatography, cleavage of the His-tag with tobacco etch virus, TEV, protease, followed by gel filtration
using the His-tagged affinity chromatography