2.1.1.157: sarcosine/dimethylglycine N-methyltransferase

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For detailed information about sarcosine/dimethylglycine N-methyltransferase, go to the full flat file.

Reaction

2 S-adenosyl-L-methionine +

Synonyms

dimethylglycine methyltransferase, DMT, DMT2, glycine sarcosine dimethylglycine methyltransferase, glycine sarcosine methyltransferase, GSDMT, GsSDMT, MXAN 3190, sarcosine dimethylglycine methyltransferas, sarcosine dimethylglycine methyltransferase, sarcosine dimethylglycine N-methyltransferase, Sdm, Sdm homologue protein, SdmA, SDMT

ECTree

     2 Transferases

         2.1 Transferring one-carbon groups

             2.1.1 Methyltransferases

                2.1.1.157 sarcosine/dimethylglycine N-methyltransferase

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Results	in table
1	Activating Compound
256	AA Sequence
3	Application
1	CAS Registry Number
11	Cloned(Commentary)
2	Cofactor
2	Crystallization (Commentary)
3	Expression
8	General Information
2	IC50 Value
23	Inhibitors
22	KM Value [mM]
3	Metals/Ions
12	Molecular Weight [Da]
32	Natural Substrates/ Products (Substrates)
18	Organism
4	Pathway
9	pH Optimum
1	pI Value
4	Protein Variants
6	Purification (Commentary)
6	Reaction
17	Reference
11	Specific Activity [micromol/min/mg]
1	Storage Stability
50	Substrates and Products (Substrate)
8	Subunits
34	Synonyms
1	Systematic Name
5	Temperature Optimum [°C]
1	Temperature Stability [°C]
4	Turnover Number [1/s]

Purification

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Purification on EC 2.1.1.157 - sarcosine/dimethylglycine N-methyltransferase

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PURIFICATION (Commentary)

ORGANISM

UNIPROT

LITERATURE

-

2 entries

by AMP affinity chromatography and ultrafiltration

Actinopolyspora halophila

-

659145

recombinant enzyme from Escherichia coli by ammonium sulfate fractionation and ion exchange chromatography to homogeneity

Aphanothece halophytica

Q83WC3

659286

recombinant enzyme from Escherichia coli by ammonium sulfate fractionation, hydrophobic and anion exchange chromatography, and ultrafiltration to homogeneity

Halorhodospira halochloris

-

657578

recombinant MpSDMT from Escherichia coli strain BL21(DE3) by nickel affinity chromatography

Methanohalophilus portucalensis

F6KV62

720836

-

Galdieria sulphuraria

-

689964

-

Methanohalophilus portucalensis

-

695924